Pseudomonas aeruginosa PAO1, PA4262 (rplD)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4262
Name
rplD
Replicon chromosome
Genomic location 4766009 - 4766611 (- strand)
Transposon Mutants 2 transposon mutants in PAO1

Cross-References

RefSeq NP_252952.1
GI 15599458
Affymetrix PA4262_rplD_at
DNASU PaCD00007850
Entrez 881754
GenBank AAG07650.1
INSDC AAG07650.1
NCBI Locus Tag PA4262
protein_id(GenBank) gb|AAG07650.1|AE004841_28|gnl|PseudoCAP|PA4262
TIGR NTL03PA04263
UniParc UPI00000C5C8C
UniProtKB Acc Q9HWD6
UniProtKB ID RL4_PSEAE
UniRef100 UniRef100_A6UZI9
UniRef50 UniRef50_A6UZI9
UniRef90 UniRef90_A6UZI9

Product

Feature Type CDS
Coding Frame 1
Product Name
50S ribosomal protein L4
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Detected by two-dimensional gel electrophoresis and mass spectral analysis(PMID:21863142).
Detected using LC-MS/MS (21.64 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 10.71
Kyte-Doolittle Hydrophobicity Value -0.209
Molecular Weight (kDa) 21.6
Isoelectric Point (pI) 10.56

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
7UNW RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-B) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.6 ELECTRON MICROSCOPY 100.0
7UNU RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-B) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.9 ELECTRON MICROSCOPY 100.0
6SPB RIBOSOME 09/01/19 Pseudomonas aeruginosa 50s ribosome from a clinical isolate with a mutation in uL6 Pseudomonas aeruginosa 2.82 ELECTRON MICROSCOPY 100.0
7UNR RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-A) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.9 ELECTRON MICROSCOPY 100.0
6SPG RIBOSOME 09/01/19 Pseudomonas aeruginosa 70s ribosome from a clinical isolate Pseudomonas aeruginosa 3.34 ELECTRON MICROSCOPY 100.0
6SPF RIBOSOME 09/01/19 Pseudomonas aeruginosa 70s ribosome from an aminoglycoside resistant clinical isolate Pseudomonas aeruginosa 2.89 ELECTRON MICROSCOPY 100.0
6SPD RIBOSOME 09/01/19 Pseudomonas aeruginosa 50s ribosome from a clinical isolate Pseudomonas aeruginosa 3.28 ELECTRON MICROSCOPY 100.0
7UNV RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-A) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.7 ELECTRON MICROSCOPY 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 591 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001448 (528 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4262
Search term: rplD

Human Homologs

Ensembl 110, assembly GRCh38.p14
mitochondrial ribosomal protein L4 [Source:HGNC Symbol;Acc:HGNC:14276]
E-value: 1.9e-10
Percent Identity: 28.5
Ensembl 110, assembly GRCh38.p14
mitochondrial ribosomal protein L4 [Source:HGNC Symbol;Acc:HGNC:14276]
E-value: 1.9e-10
Percent Identity: 28.5
Ensembl 110, assembly GRCh38.p14
mitochondrial ribosomal protein L4 [Source:HGNC Symbol;Acc:HGNC:14276]
E-value: 1.9e-10
Percent Identity: 28.5

References

Structure of the Escherichia coli S10 ribosomal protein operon.
Zurawski G, Zurawski SM
Nucleic Acids Res 1985 Jun 25;13(12):4521-6
PubMed ID: 3892488
Autogenous control of the S10 ribosomal protein operon of Escherichia coli: genetic dissection of transcriptional and posttranscriptional regulation.
Freedman LP, Zengel JM, Archer RH, Lindahl L
Proc Natl Acad Sci U S A 1987 Sep;84(18):6516-20
PubMed ID: 2442760
Ribosomal protein L4 from Escherichia coli utilizes nonidentical determinants for its structural and regulatory functions.
Li X, Lindahl L, Zengel JM
RNA 1996 Jan;2(1):24-37
PubMed ID: 8846294
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344