Pseudomonas aeruginosa PAO1, PA4273 (rplA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4273
Name
rplA
Replicon chromosome
Genomic location 4781985 - 4782680 (- strand)

Cross-References

RefSeq NP_252963.1
GI 15599469
Affymetrix PA4273_rplA_at
DNASU PaCD00007855
Entrez 881704
GenBank AAG07661.1
INSDC AAG07661.1
NCBI Locus Tag PA4273
protein_id(GenBank) gb|AAG07661.1|AE004843_3|gnl|PseudoCAP|PA4273
TIGR NTL03PA04274
UniParc UPI00000C5C8E
UniProtKB Acc Q9HWC6
UniProtKB ID RL1_PSEAE
UniRef100 UniRef100_A6UZH8
UniRef50 UniRef50_Q6N4R5
UniRef90 UniRef90_C1DKK2

Product

Feature Type CDS
Coding Frame 1
Product Name
50S ribosomal protein L1
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (24.23 kDa, PMID:21751344)
Charge (pH 7) 8.23
Kyte-Doolittle Hydrophobicity Value -0.077
Molecular Weight (kDa) 24.2
Isoelectric Point (pI) 10.28

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 662 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001438 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4273
Search term: rplA

Human Homologs

References

The primary structure of protein L1 from the large ribosomal subunit of Escherichia coli.
Brauer D, Ochsner I
FEBS Lett 1978 Dec 15;96(2):317-21
PubMed ID: 365581
Nucleotide sequence of the ribosomal protein gene cluster adjacent to the gene for RNA polymerase subunit beta in Escherichia coli.
Post LE, Strycharz GD, Nomura M, Lewis H, Dennis PP
Proc Natl Acad Sci U S A 1979 Apr;76(4):1697-701
PubMed ID: 377281
Translational regulation of the L11 ribosomal protein operon of Escherichia coli: mutations that define the target site for repression by L1.
Thomas MS, Nomura M
Nucleic Acids Res 1987 Apr 10;15(7):3085-96
PubMed ID: 3104883
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344