Pseudomonas aeruginosa PAO1, PA0085 (hcp1)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
Locus Tag
Replicon chromosome
Genomic location 103350 - 103838 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs


RefSeq NP_248775.1
GI 15595283
Affymetrix PA0085_at
DNASU PaCD00006037
Entrez 879446
GenBank AAG03475.1
INSDC AAG03475.1
NCBI Locus Tag PA0085
protein_id(GenBank) gb|AAG03475.1|AE004447_8|gnl|PseudoCAP|PA0085
UniParc UPI00000C4F26
UniProtKB Acc Q9I747
UniRef100 UniRef100_Q9I747
UniRef50 UniRef50_Q9I747
UniRef90 UniRef90_Q9I747


Feature Type CDS
Coding Frame 1
Product Name
Product Name Confidence: Class 1
Evidence for Translation
Detected by two-dimensional gel electrophoresis, mass spectral analysis and protein mass mapping (PMID:21863142).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -0.32
Kyte-Doolittle Hydrophobicity Value -0.415
Molecular Weight (kDa) 17.4
Isoelectric Point (pI) 6.80

Subcellular localization

Individual Mappings
Localization Confidence PMID
Extracellular Class 3
Extracellular Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6AP1 TRANSPORT PROTEIN 08/16/17 Vps4p-Vta1p complex with peptide binding to the central pore of Vps4p Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1); Saccharomyces cerevisiae (strain ATCC 204508 / S288c); SYNTHETIC CONSTRUCT 3.2 ELECTRON MICROSCOPY 99.4
4KSR PROTEIN TRANSPORT 05/17/13 Crystal Structure of the Vibrio cholerae ATPase GspE Hexamer Pseudomonas aeruginosa; Vibrio cholerae O1 4.2 X-RAY DIFFRACTION 100.0
1Y12 structural genomics, unknown function 11/16/04 Structure of a hemolysin-coregulated protein from Pseudomonas aeruginosa Pseudomonas aeruginosa PAO1 1.95 X-RAY DIFFRACTION 100.0
7O42 MEMBRANE PROTEIN 04/04/21 TrwK/VirB4unbound trimer of dimers complex (with Hcp1) from the R388 type IV secretion system determined by cryo-EM. Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1); Salmonella dublin 4.1 ELECTRON MICROSCOPY 98.1
4KSS PROTEIN TRANSPORT 05/17/13 Crystal Structure of Vibrio cholerae ATPase GspsE Hexamer Pseudomonas aeruginosa; Vibrio cholerae O1 7.58 X-RAY DIFFRACTION 100.0

Virulence Evidence

Source 1
Database VFDB : VF0334
Category Hcp secretion island-1 encoded type VI secretion system (H-T6SS)
Evidence ECO:0000305
curator inference used in manual assertion
Host Organism
Reference 16763151

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 75 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000084 (750 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA0085
Search term: hcp1
Search term: Hcp1

Human Homologs


A virulence locus of Pseudomonas aeruginosa encodes a protein secretion apparatus.
Mougous JD, Cuff ME, Raunser S, Shen A, Zhou M, Gifford CA, Goodman AL, Joachimiak G, Ordoñez CL, Lory S, Walz T, Joachimiak A, Mekalanos JJ
Science 2006 Jun 9;312(5779):1526-30
PubMed ID: 16763151
A type VI secretion system of Pseudomonas aeruginosa targets a toxin to bacteria.
Hood RD, Singh P, Hsu F, Güvener T, Carl MA, Trinidad RR, Silverman JM, Ohlson BB, Hicks KG, Plemel RL, Li M, Schwarz S, Wang WY, Merz AJ, Goodlett DR, Mougous JD
Cell Host Microbe 2010 Jan 21;7(1):25-37
PubMed ID: 20114026
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142