Pseudomonas aeruginosa PAO1, PA0086 (tagJ1)
Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
Gene Feature Overview
| Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
| Locus Tag |
PA0086
|
| Name |
tagJ1
Synonym: hsiE1 |
| Replicon | chromosome |
| Genomic location | 104006 - 104851 (+ strand) |
| Transposon Mutants | 1 transposon mutants in PAO1 |
| Comment | Hcp Secretion Island-I-encoded type VI secretion system (H1-T6SS) |
Cross-References
| RefSeq | NP_248776.1 |
| GI | 15595284 |
| Affymetrix | PA0086_at |
| DNASU | PaCD00006038 |
| Entrez | 879504 |
| GenBank | AAG03476.1 |
| INSDC | AAG03476.1 |
| NCBI Locus Tag | PA0086 |
| protein_id(GenBank) | gb|AAG03476.1|AE004447_9|gnl|PseudoCAP|PA0086 |
| TIGR | NTL03PA00087 |
| UniParc | UPI00000C4F27 |
| UniProtKB Acc | Q9I746 |
| UniProtKB ID | Q9I746_PSEAE |
| UniRef100 | UniRef100_Q9I746 |
| UniRef50 | UniRef50_A1KCF9 |
| UniRef90 | UniRef90_Q9I746 |
Product
| Feature Type | CDS |
| Coding Frame | 1 |
| Product Name |
TagJ1
|
| Synonyms | |
| Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
| Charge (pH 7) | -12.54 |
| Kyte-Doolittle Hydrophobicity Value | 0.019 |
| Molecular Weight (kDa) | 30.4 |
| Isoelectric Point (pI) | 4.41 |
Subcellular localization
| Individual Mappings | |
| Additional evidence for subcellular localization |
PDB 3D Structures
| Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
| 4UQZ | PROTEIN TRANSPORT | 06/25/14 | Coevolution of the ATPase ClpV, the TssB-TssC Sheath and the Accessory HsiE Protein Distinguishes Two Type VI Secretion Classes | PSEUDOMONAS AERUGINOSA PAO1 | 1.599 | X-RAY DIFFRACTION | 100.0 |
| 4UQY | PROTEIN TRANSPORT | 06/25/14 | Coevolution of the ATPase ClpV, the TssB-TssC Sheath and the Accessory HsiE Protein Distinguishes Two Type VI Secretion Classes | PSEUDOMONAS AERUGINOSA PAO1; SYNTHETIC CONSTRUCT | 1.6 | X-RAY DIFFRACTION | 100.0 |
| 4UQX | PROTEIN TRANSPORT | 06/25/14 | Coevolution of the ATPase ClpV, the TssB-TssC Sheath and the Accessory HsiE Protein Distinguishes Two Type VI Secretion Classes | PSEUDOMONAS AERUGINOSA PAO1 | 1.2 | X-RAY DIFFRACTION | 100.0 |
Virulence Evidence
| Source 1 | |
| Database | VFDB : VF0334 |
| Category | Hcp secretion island-1 encoded type VI secretion system (H-T6SS) |
| Evidence |
ECO:0000305
curator inference used in manual assertion
|
| Host Organism | |
| Notes | |
| Reference | 16763151 |
Pathogen Association Analysis
| Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 44 genera
|
Orthologs/Comparative Genomics
| Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
| Pseudomonas Ortholog Group |
POG000085 (304 members) |
| Putative Inparalogs | None Found |
Interactions
| STRING database | Search for predicted protein-protein interactions using:
Search term: PA0086
Search term: tagJ1
Search term: TagJ1
|
Human Homologs
References
|
A type VI secretion system of Pseudomonas aeruginosa targets a toxin to bacteria.
Hood RD, Singh P, Hsu F, Güvener T, Carl MA, Trinidad RR, Silverman JM, Ohlson BB, Hicks KG, Plemel RL, Li M, Schwarz S, Wang WY, Merz AJ, Goodlett DR, Mougous JD
Cell Host Microbe 2010 Jan 21;7(1):25-37
PubMed ID: 20114026
|
|
In vivo expression technology identifies a type VI secretion system locus in Burkholderia pseudomallei that is induced upon invasion of macrophages.
Shalom G, Shaw JG, Thomas MS
Microbiology (Reading) 2007 Aug;153(Pt 8):2689-2699
PubMed ID: 17660433
|
|
RsmA and AmrZ orchestrate the assembly of all three type VI secretion systems in Pseudomonas aeruginosa.
Allsopp LP, Wood TE, Howard SA, Maggiorelli F, Nolan LM, Wettstadt S, Filloux A
Proc Natl Acad Sci U S A 2017 Jul 18;114(29):7707-7712
PubMed ID: 28673999
|
|
Coevolution of the ATPase ClpV, the sheath proteins TssB and TssC, and the accessory protein TagJ/HsiE1 distinguishes type VI secretion classes.
Förster A, Planamente S, Manoli E, Lossi NS, Freemont PS, Filloux A
J Biol Chem 2014 Nov 21;289(47):33032-43
PubMed ID: 25305017
|
|
The archetype Pseudomonas aeruginosa proteins TssB and TagJ form a novel subcomplex in the bacterial type VI secretion system.
Lossi NS, Manoli E, Simpson P, Jones C, Hui K, Dajani R, Coulthurst SJ, Freemont P, Filloux A
Mol Microbiol 2012 Oct;86(2):437-56
PubMed ID: 22906320
|