Pseudomonas aeruginosa PAO1, PA0093 (tse6)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0093
Name
tse6
Replicon chromosome
Genomic location 113303 - 114595 (- strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 4 transposon mutants in orthologs

Cross-References

RefSeq NP_248783.1
GI 15595291
Affymetrix PA0093_at
Entrez 880654
GenBank AAG03483.1
INSDC AAG03483.1
NCBI Locus Tag PA0093
protein_id(GenBank) gb|AAG03483.1|AE004448_2|gnl|PseudoCAP|PA0093
TIGR NTL03PA00094
UniParc UPI00000C4F2E
UniProtKB Acc Q9I739
UniProtKB ID Q9I739_PSEAE
UniRef100 UniRef100_Q9I739
UniRef50 UniRef50_Q9I739
UniRef90 UniRef90_Q9I739

Product

Feature Type CDS
Coding Frame 1
Product Name
Tse6
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 4.38
Kyte-Doolittle Hydrophobicity Value 0.068
Molecular Weight (kDa) 44.7
Isoelectric Point (pI) 8.94

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6OX6 TRANSFERASE 05/13/19 Crystal structure of the complex between the Type VI effector Tas1 and its immunity protein Pseudomonas aeruginosa 2.17 X-RAY DIFFRACTION 100.0
4ZV4 TRANSLATION 05/18/15 Structure of Tse6 in complex with EF-Tu Pseudomonas aeruginosa; Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) 3.504 X-RAY DIFFRACTION 100.0
4ZV0 PROTEIN BINDING 05/18/15 Structure of Tse6 in complex with Tsi6 Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) 1.401 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 26 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000092 (1670 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0093
Search term: tse6
Search term: Tse6

Human Homologs

References

Genetically distinct pathways guide effector export through the type VI secretion system.
Whitney JC, Beck CM, Goo YA, Russell AB, Harding BN, De Leon JA, Cunningham DA, Tran BQ, Low DA, Goodlett DR, Hayes CS, Mougous JD
Mol Microbiol 2014 May;92(3):529-42
PubMed ID: 24589350
An interbacterial NAD(P)(+) glycohydrolase toxin requires elongation factor Tu for delivery to target cells.
Whitney JC, Quentin D, Sawai S, LeRoux M, Harding BN, Ledvina HE, Tran BQ, Robinson H, Goo YA, Goodlett DR, Raunser S, Mougous JD
Cell 2015 Oct 22;163(3):607-19
PubMed ID: 26456113