Pseudomonas aeruginosa PAO1, PA0169 (siaD)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0169
Name
siaD
Replicon chromosome
Genomic location 192965 - 193672 (- strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 6 transposon mutants in orthologs
CommentInvolved in postranscriptional regulation of CupA fimbriae via transcriptional activation of rsmZ
CommentInvolved in surfactant-induced (SDS) aggregation.

Cross-References

RefSeq NP_248859.1
GI 15595367
Affymetrix PA0169_at
Entrez 879346
GenBank AAG03559.1
INSDC AAG03559.1
NCBI Locus Tag PA0169
protein_id(GenBank) gb|AAG03559.1|AE004454_11|gnl|PseudoCAP|PA0169
TIGR NTL03PA00170
UniParc UPI00000C4F78
UniProtKB Acc Q9I6W3
UniProtKB ID Q9I6W3_PSEAE
UniRef100 UniRef100_Q9I6W3
UniRef50 UniRef50_Q9I6W3
UniRef90 UniRef90_Q9I6W3

Product

Feature Type CDS
Coding Frame 1
Product Name
SiaD
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 4.91
Kyte-Doolittle Hydrophobicity Value -0.539
Molecular Weight (kDa) 26.8
Isoelectric Point (pI) 9.49

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
7E6G DE NOVO PROTEIN 02/22/21 Crystal structure of diguanylate cyclase SiaD in complex with its activator SiaC from Pseudomonas aeruginosa Pseudomonas aeruginosa 2.65 X-RAY DIFFRACTION 95.7

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 454 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000167 (2758 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0169
Search term: siaD
Search term: SiaD

Human Homologs

References

Detergent-induced cell aggregation in subpopulations of Pseudomonas aeruginosa as a preadaptive survival strategy.
Klebensberger J, Lautenschlager K, Bressler D, Wingender J, Philipp B
Environ Microbiol 2007 Sep;9(9):2247-59
PubMed ID: 17686022
SiaA and SiaD are essential for inducing autoaggregation as a specific response to detergent stress in Pseudomonas aeruginosa.
Klebensberger J, Birkenmaier A, Geffers R, Kjelleberg S, Philipp B
Environ Microbiol 2009 Dec;11(12):3073-86
PubMed ID: 19638175
SiaA/D Interconnects c-di-GMP and RsmA Signaling to Coordinate Cellular Aggregation of Pseudomonas aeruginosa in Response to Environmental Conditions.
Colley B, Dederer V, Carnell M, Kjelleberg S, Rice SA, Klebensberger J
Front Microbiol 2016;7:179
PubMed ID: 26955366