Pseudomonas aeruginosa PAO1, PA1074 (braC)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
Locus Tag
Replicon chromosome
Genomic location 1161856 - 1162977 (- strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs


RefSeq NP_249765.1
GI 15596271
Affymetrix PA1074_braC_at
Entrez 878501
GenBank AAG04463.1
INSDC AAG04463.1
NCBI Locus Tag PA1074
protein_id(GenBank) gb|AAG04463.1|AE004539_5|gnl|PseudoCAP|PA1074
UniParc UPI0000126AB6
UniProtKB Acc P21175
UniRef100 UniRef100_P21175
UniRef50 UniRef50_P21175
UniRef90 UniRef90_P21175


Feature Type CDS
Coding Frame 1
Product Name
branched-chain amino acid transport protein BraC
Product Name Confidence: Class 1
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (39.77 kDa, PMID:21751344)
Charge (pH 7) -4.09
Kyte-Doolittle Hydrophobicity Value -0.155
Molecular Weight (kDa) 39.8
Isoelectric Point (pI) 5.53

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 3
Outer Membrane Vesicle Class 1
Periplasmic Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 414 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001036 (536 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA1074
Search term: braC

Human Homologs


Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Cloning and nucleotide sequence of braC, the structural gene for the leucine-, isoleucine-, and valine-binding protein of Pseudomonas aeruginosa PAO.
Hoshino T, Kose K
J Bacteriol 1989 Nov;171(11):6300-6
PubMed ID: 2509433
Cloning, nucleotide sequences, and identification of products of the Pseudomonas aeruginosa PAO bra genes, which encode the high-affinity branched-chain amino acid transport system.
Hoshino T, Kose K
J Bacteriol 1990 Oct;172(10):5531-9
PubMed ID: 2120183
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344