Pseudomonas aeruginosa PAO1, PA1086 (flgK)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1086
Name
flgK
Replicon chromosome
Genomic location 1172163 - 1174214 (+ strand)
Transposon Mutants 7 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_249777.1
GI 15596283
Affymetrix PA1086_flgK_at
Entrez 878191
GenBank AAG04475.1
INSDC AAG04475.1
NCBI Locus Tag PA1086
protein_id(GenBank) gb|AAG04475.1|AE004539_17|gnl|PseudoCAP|PA1086
TIGR NTL03PA01087
UniParc UPI00000C5240
UniProtKB Acc Q9I4P3
UniProtKB ID Q9I4P3_PSEAE
UniRef100 UniRef100_Q9I4P3
UniRef50 UniRef50_M4WY12
UniRef90 UniRef90_Q9I4P3

Product

Feature Type CDS
Coding Frame 1
Product Name
flagellar hook-associated protein 1 FlgK
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Detected using LC-MS/MS (71.73 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -16.50
Kyte-Doolittle Hydrophobicity Value -0.287
Molecular Weight (kDa) 71.7
Isoelectric Point (pI) 4.45

Subcellular localization

Individual Mappings
Localization Confidence PMID
Flagellar
Note: This is based on PSORTb V3.0 secondary localization prediction
Class 3
Outer Membrane Vesicle Class 1
Extracellular Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database VFDB : VF0273
Category Flagella
Evidence
Host Organism
Notes
Reference 14617143

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 308 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001048 (534 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1086
Search term: flgK

Human Homologs

References

Stoichiometric analysis of the flagellar hook-(basal-body) complex of Salmonella typhimurium.
Jones CJ, Macnab RM, Okino H, Aizawa S
J Mol Biol 1990 Mar 20;212(2):377-87
PubMed ID: 2181149
Flagellar hook and hook-associated proteins of Salmonella typhimurium and their relationship to other axial components of the flagellum.
Homma M, DeRosier DJ, Macnab RM
J Mol Biol 1990 Jun 20;213(4):819-32
PubMed ID: 2193164
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344