Pseudomonas aeruginosa PAO1, PA1101 (fliF)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1101
Name
fliF
Replicon chromosome
Genomic location 1192405 - 1194201 (+ strand)
Transposon Mutants 5 transposon mutants in PAO1
Transposon Mutants in orthologs 3 transposon mutants in orthologs

Cross-References

RefSeq NP_249792.1
GI 15596298
Affymetrix PA1101_fliF_at
Entrez 881927
GenBank AAG04490.1
INSDC AAG04490.1
NCBI Locus Tag PA1101
protein_id(GenBank) gb|AAG04490.1|AE004540_10|gnl|PseudoCAP|PA1101
TIGR NTL03PA01102
UniParc UPI000012A9A7
UniProtKB Acc Q51463
UniProtKB ID FLIF_PSEAE
UniRef100 UniRef100_Q51463
UniRef50 UniRef50_Q51463
UniRef90 UniRef90_Q51463

Product

Feature Type CDS
Coding Frame 1
Product Name
Flagella M-ring outer membrane protein precursor
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -13.25
Kyte-Doolittle Hydrophobicity Value -0.266
Molecular Weight (kDa) 64.0
Isoelectric Point (pI) 4.74

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Outer Membrane Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database VFDB : VF0273
Category Flagella
Evidence
Host Organism
Notes
Reference 14617143

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 307 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001062 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1101
Search term: fliF

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Cloning and characterization of Pseudomonas aeruginosa fliF, necessary for flagellar assembly and bacterial adherence to mucin.
Arora SK, Ritchings BW, Almira EC, Lory S, Ramphal R
Infect Immun 1996 Jun;64(6):2130-6
PubMed ID: 8675317