Pseudomonas aeruginosa PAO1, PA1121 (yfiR)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
Locus Tag
Replicon chromosome
Genomic location 1214502 - 1215074 (- strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs


RefSeq NP_249812.1
GI 15596318
Affymetrix PA1121_at
DNASU PaCD00006517
Entrez 877671
GenBank AAG04510.1
INSDC AAG04510.1
NCBI Locus Tag PA1121
protein_id(GenBank) gb|AAG04510.1|AE004542_6|gnl|PseudoCAP|PA1121
UniParc UPI00000C525C
UniProtKB Acc Q9I4L4
UniRef100 UniRef100_Q9I4L4
UniRef50 UniRef50_W0YNF8
UniRef90 UniRef90_W0YNF8


Feature Type CDS
Coding Frame 1
Product Name
Product Name Confidence: Class 1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 4.53
Kyte-Doolittle Hydrophobicity Value 0.040
Molecular Weight (kDa) 20.7
Isoelectric Point (pI) 8.61

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 3
Periplasmic Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4YNA TRANSCRIPTION 03/09/15 Oxidized YfiR Pseudomonas aeruginosa PAO1 3.2 X-RAY DIFFRACTION 99.4
5EB2 TRANSCRIPTION 10/17/15 Trp-bound YfiR Pseudomonas aeruginosa PAO1 2.709 X-RAY DIFFRACTION 99.4
6IKK SIGNALING PROTEIN 10/16/18 Crystal structure of YfiB(L43P) in complex with YfiR Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2.19 X-RAY DIFFRACTION 100.0
4ZHY TRANSCRIPTION/SIGNALING PROTEIN 04/27/15 Crystal structure of a bacterial signalling complex Pseudomonas aeruginosa PAO1 1.969 X-RAY DIFFRACTION 99.5
4XJY SIGNALING PROTEIN 01/09/15 Periplasmic repressor protein YfiR Pseudomonas aeruginosa PAO1 1.8 X-RAY DIFFRACTION 100.0
5EB1 MEMBRANE PROTEIN/TRANSCRIPTION 10/17/15 the YfiB-YfiR complex Pseudomonas aeruginosa PAO1 1.8 X-RAY DIFFRACTION 99.4
5Y61 SIGNALING PROTEIN 08/10/17 YfiB-YfiR complexed with GMP Pseudomonas aeruginosa 2.99 X-RAY DIFFRACTION 99.4
5EB3 TRANSCRIPTION 10/17/15 VB6-bound protein Pseudomonas aeruginosa PAO1 2.4 X-RAY DIFFRACTION 99.4
5Y62 SIGNALING PROTEIN 08/10/17 YfiR complexed with GMP Pseudomonas aeruginosa 3 X-RAY DIFFRACTION 99.4
4YN7 TRANSCRIPTION 03/09/15 Non-oxidized YfiR Pseudomonas aeruginosa PAO1 2.6 X-RAY DIFFRACTION 99.4
4ZHU TRANSCRIPTION 04/27/15 Crystal structure of a bacterial repressor protein Pseudomonas aeruginosa PAO1 2.3968 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 38 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001081 (444 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA1121
Search term: yfiR
Search term: YfiR

Human Homologs


Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
YfiBNR mediates cyclic di-GMP dependent small colony variant formation and persistence in Pseudomonas aeruginosa.
Malone JG, Jaeger T, Spangler C, Ritz D, Spang A, Arrieumerlou C, Kaever V, Landmann R, Jenal U
PLoS Pathog 2010 Mar 12;6(3):e1000804
PubMed ID: 20300602