Pseudomonas aeruginosa PAO1, PA1122

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1122
Name
Synonym: def
Synonym: fms
Synonym: pdf
Replicon chromosome
Genomic location 1215284 - 1215727 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_249813
GI 15596319
Affymetrix PA1122_at
Entrez 878310
GenBank AAG04511.1
NCBI Locus Tag PA1122
protein_id(GenBank) gb|AAG04511.1|AE004542_7|gnl|PseudoCAP|PA1122
TIGR NTL03PA01123
UniProtKB Acc Q9I4L3
UniProtKB ID Q9I4L3_PSEAE

Product

Feature Type CDS
Coding Frame 1
Product Name
putative peptide deformylase
Product Name Confidence: Class 3
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -4.34
Kyte-Doolittle Hydrophobicity Value -0.307
Molecular Weight (kDa) 16.3
Isoelectric Point (pI) 4.99

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6JFA HYDROLASE 02/08/19 Met-Ala-Ser bound crystal structure of class I type b peptide deformylase from Pseudomonas aeruginosa Pseudomonas aeruginosa; SYNTHETIC CONSTRUCT 1.93 X-RAY DIFFRACTION 98.4
6JFD HYDROLASE 02/08/19 K1U bound crystal structure of class I type b peptide deformylase from Pseudomonas aeruginosa Pseudomonas aeruginosa 2.4 X-RAY DIFFRACTION 98.4
6JFN HYDROLASE 02/11/19 K4U bound crystal structure of class I type b peptide deformylase from Pseudomonas aeruginosa Pseudomonas aeruginosa 2.04 X-RAY DIFFRACTION 98.4
6JFC HYDROLASE 02/08/19 Actinonin bound crystal structure of class I type b peptide deformylase from Pseudomonas aeruginosa Pseudomonas aeruginosa 2.04 X-RAY DIFFRACTION 98.4
6JFF HYDROLASE 02/08/19 K3U bound crystal structure of class I type b peptide deformylase from Pseudomonas aeruginosa Pseudomonas aeruginosa 2.1 X-RAY DIFFRACTION 98.4
6JF9 HYDROLASE 02/08/19 Apo crystal structure of class I type b peptide deformylase from Pseudomonas aeruginosa Pseudomonas aeruginosa 1.7 X-RAY DIFFRACTION 98.4
6JFE HYDROLASE 02/08/19 K2U bound crystal structure of class I type b peptide deformylase from Pseudomonas aeruginosa Pseudomonas aeruginosa 2.1 X-RAY DIFFRACTION 98.4

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 568 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001082 (483 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1122

Human Homologs

References

Evidence that peptide deformylase and methionyl-tRNA(fMet) formyltransferase are encoded within the same operon in Escherichia coli.
Meinnel T, Blanquet S
J Bacteriol 1993 Dec;175(23):7737-40
PubMed ID: 8244948
A new subclass of the zinc metalloproteases superfamily revealed by the solution structure of peptide deformylase.
Meinnel T, Blanquet S, Dardel F
J Mol Biol 1996 Sep 27;262(3):375-86
PubMed ID: 8845003