Pseudomonas aeruginosa PAO1, PA1150 (pys2)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1150
Name
pys2
Replicon chromosome
Genomic location 1243594 - 1245663 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_249841.1
GI 15596347
Affymetrix PA1150_pys2_at
Entrez 878080
GenBank AAG04539.1
INSDC AAG04539.1
NCBI Locus Tag PA1150
protein_id(GenBank) gb|AAG04539.1|AE004545_2|gnl|PseudoCAP|PA1150
TIGR NTL03PA01151
UniParc UPI0000165EC2
UniProtKB Acc Q06584
UniProtKB ID PYS2_PSEAE
UniRef100 UniRef100_Q06584
UniRef50 UniRef50_Q06584
UniRef90 UniRef90_Q06584

Product

Feature Type CDS
Coding Frame 1
Product Name
pyocin S2
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Detected using LC-MS/MS (73.85 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 9.71
Kyte-Doolittle Hydrophobicity Value -0.360
Molecular Weight (kDa) 73.9
Isoelectric Point (pI) 9.53

Subcellular localization

Individual Mappings
Localization Confidence PMID
Extracellular Class 3
Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4QKO ANTIMICROBIAL PROTEIN 06/07/14 The Crystal Structure of the Pyocin S2 Nuclease Domain, Immunity Protein Complex at 1.8 Angstroms Pseudomonas aeruginosa 1.8 X-RAY DIFFRACTION 100.0
5ODW PROTEIN TRANSPORT 07/06/17 Structure of the FpvAI-pyocin S2 complex Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2.8 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 18 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000948 (428 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1150
Search term: pys2
Search term: pyocin S2

Human Homologs

References

Purification of the pyocin S2 complex from Pseudomonas aeruginosa PAO1: analysis of DNase activity.
Seo Y, Galloway DR
Biochem Biophys Res Commun 1990 Oct 30;172(2):455-61
PubMed ID: 2122894
Molecular structures and functions of pyocins S1 and S2 in Pseudomonas aeruginosa.
Sano Y, Matsui H, Kobayashi M, Kageyama M
J Bacteriol 1993 May;175(10):2907-16
PubMed ID: 8491711
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344