Pseudomonas aeruginosa PAO1, PA1179 (phoP)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA1179
Name	phoP
Replicon	chromosome
Genomic location	1277688 - 1278365 (+ strand)
Transposon Mutants	3 transposon mutants in PAO1
Transposon Mutants in orthologs	1 transposon mutants in orthologs
Comment	PhoP-PhoQ two-component regulatory system contributes to bacterial tolerance to polymyxin B by directly regulating genes involved in LPS modification and membrane integrity maintenance[PMID:33572426]
Comment	PhoP directly controls the expression of PA14_46900, PA14_50740 and PA14_52340, and the operons of PA14_11970-PA14_11960 and PA14_52350-PA14_52370 [PMID:33572426]

Cross-References

RefSeq	NP_249870.1
GI	15596376
Affymetrix	PA1179_phoP_at
DNASU	PaCD00006544
Entrez	879194
GenBank	AAG04568.1
INSDC	AAG04568.1
NCBI Locus Tag	PA1179
protein_id(GenBank)	gb|AAG04568.1|AE004548_2|gnl|PseudoCAP|PA1179
TIGR	NTL03PA01180
UniParc	UPI00000C5290
UniProtKB Acc	Q9I4F9
UniProtKB ID	Q9I4F9_PSEAE
UniRef100	UniRef100_W0YNX6
UniRef50	UniRef50_Q0C2L9
UniRef90	UniRef90_A6V919

Product

Feature Type	CDS
Coding Frame	1
Product Name	two-component response regulator PhoP Product Name Confidence: Class 2
Synonyms
Evidence for Translation	Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7)	-7.37
Kyte-Doolittle Hydrophobicity Value	-0.267
Molecular Weight (kDa)	25.7
Isoelectric Point (pI)	5.21

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Class 2 Cytoplasmic Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 624 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG001137 (524 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA1179 Search term: phoP Search term: two-component response regulator PhoP

Human Homologs

References

PhoP-PhoQ homologues in Pseudomonas aeruginosa regulate expression of the outer-membrane protein OprH and polymyxin B resistance. Macfarlane EL, Kwasnicka A, Ochs MM, Hancock RE Mol Microbiol 1999 Oct;34(2):305-16 PubMed ID: 10564474

Specific lipopolysaccharide found in cystic fibrosis airway Pseudomonas aeruginosa. Ernst RK, Yi EC, Guo L, Lim KB, Burns JL, Hackett M, Miller SI Science 1999 Nov 19;286(5444):1561-5 PubMed ID: 10567263

Salmonella typhimurium phoP virulence gene is a transcriptional regulator. Groisman EA, Chiao E, Lipps CJ, Heffron F Proc Natl Acad Sci U S A 1989 Sep;86(18):7077-81 PubMed ID: 2674945

PhoQ mutations promote lipid A modification and polymyxin resistance of Pseudomonas aeruginosa found in colistin-treated cystic fibrosis patients. Miller AK, Brannon MK, Stevens L, Johansen HK, Selgrade SE, Miller SI, Høiby N, Moskowitz SM Antimicrob Agents Chemother 2011 Dec;55(12):5761-9 PubMed ID: 21968359

Identification of Novel PhoP-PhoQ Regulated Genes That Contribute to Polymyxin B Tolerance in Pseudomonas aeruginosa. Yang B, Liu C, Pan X, Fu W, Fan Z, Jin Y, Bai F, Cheng Z, Wu W Microorganisms 2021 Feb 9;9(2) PubMed ID: 33572426