Pseudomonas aeruginosa PAO1, PA1180 (phoQ)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
Locus Tag
Replicon chromosome
Genomic location 1278362 - 1279708 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentPhoP-PhoQ two-component regulatory system contributes to bacterial tolerance to polymyxin B by directly regulating genes involved in LPS modification and membrane integrity maintenance[PMID:33572426]


RefSeq NP_249871.1
GI 15596377
Affymetrix PA1180_phoQ_at
Entrez 879187
GenBank AAG04569.1
INSDC AAG04569.1
NCBI Locus Tag PA1180
protein_id(GenBank) gb|AAG04569.1|AE004548_3|gnl|PseudoCAP|PA1180
UniParc UPI00000C5291
UniProtKB Acc Q9I4F8
UniRef100 UniRef100_Q9I4F8
UniRef50 UniRef50_Q4K876
UniRef90 UniRef90_Q9I4F8


Feature Type CDS
Coding Frame 1
Product Name
two-component sensor PhoQ
Product Name Confidence: Class 2
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (50.28 kDa, PMID:21751344)
Charge (pH 7) -4.90
Kyte-Doolittle Hydrophobicity Value -0.204
Molecular Weight (kDa) 50.2
Isoelectric Point (pI) 6.09

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database PseudoCAP
Evidence ECO:0000314
direct assay evidence used in manual assertion
Host Organism Lactuca sativa
Infection model Lettuce Infection model
Reference 19246741

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 601 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001138 (522 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA1180
Search term: phoQ

Human Homologs


A two-component regulatory system (phoP phoQ) controls Salmonella typhimurium virulence.
Miller SI, Kukral AM, Mekalanos JJ
Proc Natl Acad Sci U S A 1989 Jul;86(13):5054-8
PubMed ID: 2544889
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
PhoQ mutations promote lipid A modification and polymyxin resistance of Pseudomonas aeruginosa found in colistin-treated cystic fibrosis patients.
Miller AK, Brannon MK, Stevens L, Johansen HK, Selgrade SE, Miller SI, Høiby N, Moskowitz SM
Antimicrob Agents Chemother 2011 Dec;55(12):5761-9
PubMed ID: 21968359
Identification of Novel PhoP-PhoQ Regulated Genes That Contribute to Polymyxin B Tolerance in Pseudomonas aeruginosa.
Yang B, Liu C, Pan X, Fu W, Fan Z, Jin Y, Bai F, Cheng Z, Wu W
Microorganisms 2021 Feb 9;9(2)
PubMed ID: 33572426