Pseudomonas aeruginosa PAO1, PA1204

Cytoplasmic Membrane
Outer Membrane
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
Locus Tag
Synonym: yieF
Replicon chromosome
Genomic location 1303892 - 1304449 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs


RefSeq NP_249895.1
GI 15596401
Affymetrix PA1204_at
DNASU PaCD00006558
Entrez 882133
GenBank AAG04593.1
INSDC AAG04593.1
NCBI Locus Tag PA1204
protein_id(GenBank) gb|AAG04593.1|AE004550_9|gnl|PseudoCAP|PA1204
UniParc UPI00000C52A7
UniProtKB Acc Q9I4D4
UniRef100 UniRef100_Q9I4D4
UniRef50 UniRef50_Q9I4D4
UniRef90 UniRef90_Q9I4D4


Feature Type CDS
Coding Frame 1
Product Name
NAD(P)H quinone oxidoreductase
Product Name Confidence: Class 1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -0.53
Kyte-Doolittle Hydrophobicity Value -0.110
Molecular Weight (kDa) 20.2
Isoelectric Point (pI) 6.54

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
1X77 STRUCTURAL GENOMICS, UNKNOWN FUNCTION 08/13/04 Crystal structure of a NAD(P)H-dependent FMN reductase complexed with FMN Pseudomonas aeruginosa 2.7 X-RAY DIFFRACTION 99.4
1RTT Structural genomics, unknown function 12/10/03 Crystal structure determination of a putative NADH-dependent reductase using sulfur anomalous signal Pseudomonas aeruginosa 1.28 X-RAY DIFFRACTION 99.4

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 350 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001161 (402 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA1204

Human Homologs


The complete genome sequence of Escherichia coli K-12.
Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y
Science 1997 Sep 5;277(5331):1453-62
PubMed ID: 9278503
Identification of NAD(P)H quinone oxidoreductase activity in azoreductases from P. aeruginosa: azoreductases and NAD(P)H quinone oxidoreductases belong to the same FMN-dependent superfamily of enzymes.
Ryan A, Kaplan E, Nebel JC, Polycarpou E, Crescente V, Lowe E, Preston GM, Sim E
PLoS One 2014;9(6):e98551
PubMed ID: 24915188
Pseudomonas aeruginosa MdaB and WrbA are water-soluble two-electron quinone oxidoreductases with the potential to defend against oxidative stress.
Green LK, La Flamme AC, Ackerley DF
J Microbiol 2014 Sep;52(9):771-7
PubMed ID: 25085734