Pseudomonas aeruginosa PAO1, PA1221

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1221
Name
Replicon chromosome
Genomic location 1322253 - 1324109 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_249912.1
GI 15596418
Affymetrix PA1221_at
Entrez 878373
GenBank AAG04610.1
INSDC AAG04610.1
NCBI Locus Tag PA1221
protein_id(GenBank) gb|AAG04610.1|AE004551_14|gnl|PseudoCAP|PA1221
TIGR NTL03PA01222
UniParc UPI00000C52B6
UniProtKB Acc Q9I4B7
UniProtKB ID Q9I4B7_PSEAE
UniRef100 UniRef100_Q9I4B7
UniRef50 UniRef50_Q9I4B7
UniRef90 UniRef90_Q9I4B7

Product

Feature Type CDS
Coding Frame 1
Product Name
nonribosomal peptide synthetase
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) -0.80
Kyte-Doolittle Hydrophobicity Value -0.075
Molecular Weight (kDa) 67.6
Isoelectric Point (pI) 6.91

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4DG9 LIGASE/Inhibitor 01/25/12 Structure of holo-PA1221, an NRPS protein containing adenylation and PCP domains bound to vinylsulfonamide inhibitor Pseudomonas aeruginosa 2.55 X-RAY DIFFRACTION 100.0
4DG8 LIGASE 01/25/12 Structure of PA1221, an NRPS protein containing adenylation and PCP domains Pseudomonas aeruginosa 2.15 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 487 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001177 (302 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1221

Human Homologs

References

Structure of PA1221, a nonribosomal peptide synthetase containing adenylation and peptidyl carrier protein domains.
Mitchell CA, Shi C, Aldrich CC, Gulick AM
Biochemistry 2012 Apr 17;51(15):3252-63
PubMed ID: 22452656