Pseudomonas aeruginosa PAO1, PA1225

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1225
Name
Replicon chromosome
Genomic location 1327813 - 1328439 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 3 transposon mutants in orthologs
Comment

Cross-References

RefSeq NP_249916.1
GI 15596422
Affymetrix PA1225_at
DNASU PaCD00006569
Entrez 879501
GenBank AAG04614.1
INSDC AAG04614.1
NCBI Locus Tag PA1225
protein_id(GenBank) gb|AAG04614.1|AE004552_4|gnl|PseudoCAP|PA1225
TIGR NTL03PA01226
UniParc UPI00000C52BA
UniProtKB Acc Q9I4B3
UniProtKB ID Q9I4B3_PSEAE
UniRef100 UniRef100_W0YLB6
UniRef50 UniRef50_G8Q0W8
UniRef90 UniRef90_W0YLB6

Product

Feature Type CDS
Coding Frame 1
Product Name
FAD-dependent NADPH:quinone reductase
Product Name Confidence: Class 1
Synonyms probable NAD(P)H dehydrogenase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -0.15
Kyte-Doolittle Hydrophobicity Value -0.037
Molecular Weight (kDa) 22.6
Isoelectric Point (pI) 6.97

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 246 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000820 (1495 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1225

Human Homologs

Ensembl 110, assembly GRCh38.p14
N-ribosyldihydronicotinamide:quinone reductase 2 [Source:HGNC Symbol;Acc:HGNC:7856]
E-value: 7.5e-18
Percent Identity: 30.8
Ensembl 110, assembly GRCh38.p14
N-ribosyldihydronicotinamide:quinone reductase 2 [Source:HGNC Symbol;Acc:HGNC:7856]
E-value: 7.5e-18
Percent Identity: 30.8
Ensembl 110, assembly GRCh38.p14
N-ribosyldihydronicotinamide:quinone reductase 2 [Source:HGNC Symbol;Acc:HGNC:7856]
E-value: 7.5e-18
Percent Identity: 30.8

References

NAD(P)H:menadione oxidoreductase. Novel purification of enzyme cDNA and complete amino acid sequence, and gene regulation.
Robertson JA, Chen HC, Nebert DW
J Biol Chem 1986 Nov 25;261(33):15794-9
PubMed ID: 3536915
Kinetic Characterization of PA1225 from Pseudomonas aeruginosa PAO1 Reveals a New NADPH:Quinone Reductase.
Flores E, Gadda G
Biochemistry 2018 May 29;57(21):3050-3058
PubMed ID: 29715013