Pseudomonas aeruginosa PAO1, PA3147 (wbpJ)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3147
Name
wbpJ
Replicon chromosome
Genomic location 3530467 - 3531708 (- strand)
Transposon Mutants in orthologs 5 transposon mutants in orthologs

Cross-References

RefSeq NP_251837.1
GI 15598343
Affymetrix PA3147_wbpJ_at
Entrez 882692
GenBank AAG06535.1
INSDC AAG06535.1
NCBI Locus Tag PA3147
protein_id(GenBank) gb|AAG06535.1|AE004738_9|gnl|PseudoCAP|PA3147
TIGR NTL03PA03147
UniParc UPI00000C592C
UniProtKB Acc Q9HZ80
UniProtKB ID Q9HZ80_PSEAE
UniRef100 UniRef100_K1DMM3
UniRef50 UniRef50_K1DMM3
UniRef90 UniRef90_K1DMM3

Product

Feature Type CDS
Coding Frame 1
Product Name
probable glycosyl transferase WbpJ
Product Name Confidence: Class 3
Synonyms
Evidence for Translation
Detected using LC-MS/MS (45.28 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -0.05
Kyte-Doolittle Hydrophobicity Value 0.119
Molecular Weight (kDa) 45.3
Isoelectric Point (pI) 6.99

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database VFDB : VF0085
Category LPS O-antigen (P. aeruginosa)
Evidence
Host Organism
Notes
Reference

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 255 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001311 (2094 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3147
Search term: wbpJ

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Molecular characterization of the Pseudomonas aeruginosa serotype O5 (PAO1) B-band lipopolysaccharide gene cluster.
Burrows LL, Charter DF, Lam JS
Mol Microbiol 1996 Nov;22(3):481-95
PubMed ID: 8939432
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344