Pseudomonas aeruginosa PAO1, PA3155 (wbpE)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3155
Name
wbpE
Replicon chromosome
Genomic location 3539128 - 3540207 (- strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_251845.1
GI 15598351
Affymetrix PA3155_wbpE_at
DNASU PaCD00007387
Entrez 882653
GenBank AAG06543.1
INSDC AAG06543.1
NCBI Locus Tag PA3155
protein_id(GenBank) gb|AAG06543.1|AE004739_5|gnl|PseudoCAP|PA3155
TIGR NTL03PA03155
UniParc UPI00000C592E
UniProtKB Acc Q9HZ76
UniProtKB ID WBPE_PSEAE
UniRef100 UniRef100_Q9HZ76
UniRef50 UniRef50_Q9HZ76
UniRef90 UniRef90_Q9HZ76

Product

Feature Type CDS
Coding Frame 1
Product Name
UDP-2-acetamido-2-dideoxy-d-ribo-hex-3-uluronic acid transaminase, wbpE
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -3.77
Kyte-Doolittle Hydrophobicity Value -0.004
Molecular Weight (kDa) 38.9
Isoelectric Point (pI) 5.98

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
3NYS TRANSFERASE 07/15/10 X-ray structure of the K185A mutant of WbpE (WlbE) from pseudomonas aeruginosa in complex with PLP at 1.45 angstrom resolution Pseudomonas aeruginosa 1.45 X-RAY DIFFRACTION 99.7
3NU7 TRANSFERASE 07/06/10 WbpE, an Aminotransferase from Pseudomonas aeruginosa Involved in O-antigen Assembly in Complex with the Cofactor PMP Pseudomonas aeruginosa 1.95 X-RAY DIFFRACTION 100.0
3NUB TRANSFERASE 07/06/10 WbpE, an Aminotransferase from Pseudomonas aeruginosa Involved in O-antigen Assembly in Complex with Product as the External Aldimine Pseudomonas aeruginosa 1.9 X-RAY DIFFRACTION 100.0
3NYT TRANSFERASE 07/15/10 X-ray crystal structure of the WlbE (WpbE) aminotransferase from pseudomonas aeruginosa, mutation K185A, in complex with the PLP external aldimine adduct with UDP-3-amino-2-N-acetyl-glucuronic acid, at 1.3 angstrom resolution Pseudomonas aeruginosa 1.301 X-RAY DIFFRACTION 99.7
3NU8 TRANSFERASE 07/06/10 WbpE, an Aminotransferase from Pseudomonas aeruginosa Involved in O-antigen Assembly in Complex with the Internal Aldimine Pseudomonas aeruginosa 1.5 X-RAY DIFFRACTION 100.0
3NYU TRANSFERASE 07/15/10 X-ray crystal structure of the Wbpe (WlbE) aminotransferase from pseudomonas aeruginosa as the PLP internal aldimine adduct with lysine 185 Pseudomonas aeruginosa 1.501 X-RAY DIFFRACTION 100.0

Virulence Evidence

Source 1
Database VFDB : VF0085
Category LPS O-antigen (P. aeruginosa)
Evidence
Host Organism
Notes
Reference

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 557 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002114 (610 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3155
Search term: wbpE

Human Homologs

References

Molecular characterization of the Pseudomonas aeruginosa serotype O5 (PAO1) B-band lipopolysaccharide gene cluster.
Burrows LL, Charter DF, Lam JS
Mol Microbiol 1996 Nov;22(3):481-95
PubMed ID: 8939432
Characterization of WbpB, WbpE, and WbpD and reconstitution of a pathway for the biosynthesis of UDP-2,3-diacetamido-2,3-dideoxy-D-mannuronic acid in Pseudomonas aeruginosa.
Westman EL, McNally DJ, Charchoglyan A, Brewer D, Field RA, Lam JS
J Biol Chem 2009 May 1;284(18):11854-62
PubMed ID: 19282284
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994