Pseudomonas aeruginosa PAO1, PA3156 (wbpD)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3156
Name
wbpD
Replicon chromosome
Genomic location 3540210 - 3540785 (- strand)
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_251846.1
GI 15598352
Affymetrix PA3156_wbpD_at
DNASU PaCD00007388
Entrez 882593
GenBank AAG06544.1
INSDC AAG06544.1
NCBI Locus Tag PA3156
protein_id(GenBank) gb|AAG06544.1|AE004739_6|gnl|PseudoCAP|PA3156
TIGR NTL03PA03156
UniParc UPI00000DA0F1
UniProtKB Acc G3XD01
UniProtKB ID WBPD_PSEAE
UniRef100 UniRef100_G3XD01
UniRef50 UniRef50_G3XD01
UniRef90 UniRef90_G3XD01

Product

Feature Type CDS
Coding Frame 1
Product Name
UDP-2-acetamido-3-amino-2,3-dideoxy-d-glucuronic acid N-acetyltransferase, WbpD
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 1.76
Kyte-Doolittle Hydrophobicity Value 0.066
Molecular Weight (kDa) 20.6
Isoelectric Point (pI) 7.80

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database VFDB : VF0085
Category LPS O-antigen (P. aeruginosa)
Evidence
Host Organism
Notes
Reference

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 551 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000683 (866 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3156
Search term: wbpD

Human Homologs

References

Molecular characterization of the Pseudomonas aeruginosa serotype O5 (PAO1) B-band lipopolysaccharide gene cluster.
Burrows LL, Charter DF, Lam JS
Mol Microbiol 1996 Nov;22(3):481-95
PubMed ID: 8939432
Characterization of WbpB, WbpE, and WbpD and reconstitution of a pathway for the biosynthesis of UDP-2,3-diacetamido-2,3-dideoxy-D-mannuronic acid in Pseudomonas aeruginosa.
Westman EL, McNally DJ, Charchoglyan A, Brewer D, Field RA, Lam JS
J Biol Chem 2009 May 1;284(18):11854-62
PubMed ID: 19282284