Pseudomonas aeruginosa PAO1, PA3158 (wbpB)
Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
Gene Feature Overview
| Strain |
Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest |
| Locus Tag |
PA3158
|
| Name |
wbpB
|
| Replicon | chromosome |
| Genomic location | 3542740 - 3543690 (- strand) |
| Transposon Mutants in orthologs | 1 transposon mutants in orthologs |
Cross-References
| RefSeq | NP_251848.1 |
| GI | 15598354 |
| Affymetrix | PA3158_wbpB_at |
| DNASU | PaCD00007389 |
| Entrez | 882642 |
| GenBank | AAG06546.1 |
| INSDC | AAG06546.1 |
| NCBI Locus Tag | PA3158 |
| protein_id(GenBank) | gb|AAG06546.1|AE004739_8|gnl|PseudoCAP|PA3158 |
| TIGR | NTL03PA03158 |
| UniParc | UPI00000DA0FF |
| UniProtKB Acc | G3XD23 |
| UniProtKB ID | WBPB_PSEAE |
| UniRef100 | UniRef100_G3XD23 |
| UniRef50 | UniRef50_G3XD23 |
| UniRef90 | UniRef90_G3XD23 |
Product
| Feature Type | CDS |
| Coding Frame | 1 |
| Product Name |
UDP-2-acetamido-2-deoxy-d-glucuronic acid 3-dehydrogenase, WbpB
|
| Synonyms |
UDP-2-acetamido-2-deoxy-d-glucuronic acid 3-dehydrogenase, WbpB |
| Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
| Charge (pH 7) | -3.03 |
| Kyte-Doolittle Hydrophobicity Value | -0.266 |
| Molecular Weight (kDa) | 35.7 |
| Isoelectric Point (pI) | 6.57 |
Subcellular localization
| Individual Mappings | |
| Additional evidence for subcellular localization |
AlphaFold Protein Structure Database
Available for P. aeruginosa PAO1.
| View predicted 3D structure in AlphaFold DB |
PDB 3D Structures
| Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
| 3OA2 | OXIDOREDUCTASE | 08/04/10 | Crystal structure of the WlbA (WbpB) dehydrogenase from Pseudomonas aeruginosa in complex with NAD at 1.5 angstrom resolution | Pseudomonas aeruginosa | 1.5 | X-RAY DIFFRACTION | 99.7 |
Virulence Evidence
| Source 1 | |
| Database | VFDB : VF0085 |
| Category | LPS O-antigen (P. aeruginosa) |
| Evidence |
|
| Host Organism | |
| Notes | |
| Reference | |
Pathogen Association Analysis
| Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 201 genera
|
Orthologs/Comparative Genomics
| Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
| Pseudomonas Ortholog Group |
POG000236 (924 members) |
| Putative Inparalogs | None Found |
Interactions
| STRING database | Search for predicted protein-protein interactions using:
Search term: PA3158
Search term: wbpB
|
Human Homologs
References
|
Molecular characterization of the Pseudomonas aeruginosa serotype O5 (PAO1) B-band lipopolysaccharide gene cluster.
Burrows LL, Charter DF, Lam JS
Mol. Microbiol. 1996 Nov;22(3):481-95
PubMed ID: 8939432
|
|
Characterization of WbpB, WbpE, and WbpD and reconstitution of a pathway for the biosynthesis of UDP-2,3-diacetamido-2,3-dideoxy-D-mannuronic acid in Pseudomonas aeruginosa.
Westman EL, McNally DJ, Charchoglyan A, Brewer D, Field RA, Lam JS
J. Biol. Chem. 2009 May;284(18):11854-62
PubMed ID: 19282284
|