Pseudomonas aeruginosa PAO1, PA3160 (wzz)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3160
Name
wzz
Synonym: cld
Synonym: rol
Synonym: wzz1
Replicon chromosome
Genomic location 3545881 - 3546927 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_251850.1
GI 15598356
Affymetrix PA3160_wzz_at
DNASU PaCD00007390
Entrez 879922
GenBank AAG06548.1
INSDC AAG06548.1
NCBI Locus Tag PA3160
protein_id(GenBank) gb|AAG06548.1|AE004740_1|gnl|PseudoCAP|PA3160
TIGR NTL03PA03160
UniParc UPI00000DA0DA
UniProtKB Acc G3XCY2
UniProtKB ID G3XCY2_PSEAE
UniRef100 UniRef100_G3XCY2
UniRef50 UniRef50_G3XCY2
UniRef90 UniRef90_G3XCY2

Product

Feature Type CDS
Coding Frame 1
Product Name
O-antigen chain length regulator
Product Name Confidence: Class 1
Synonyms O-antigen chain length regulator Rol
Wzz
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (39.26 kDa, PMID:21751344)
Charge (pH 7) -1.29
Kyte-Doolittle Hydrophobicity Value -0.143
Molecular Weight (kDa) 39.3
Isoelectric Point (pI) 6.34

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database VFDB : VF0085
Category LPS O-antigen (P. aeruginosa)
Evidence
Host Organism
Notes
Reference
Source 2
Database Victors : 4240
Category LPS O-antigen (P. aeruginosa)
Evidence ECO:0000015
mutant phenotype evidence
Host Organism Mus musculus
Notes
Reference 18065548

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 34 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000904 (708 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3160
Search term: wzz

Human Homologs

References

Pseudomonas aeruginosa B-band O-antigen chain length is modulated by Wzz (Ro1).
Burrows LL, Chow D, Lam JS
J Bacteriol 1997 Mar;179(5):1482-9
PubMed ID: 9045803
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
Pseudomonas aeruginosa O-antigen chain length is determined before ligation to lipid A core.
Daniels C, Griffiths C, Cowles B, Lam JS
Environ Microbiol 2002 Dec;4(12):883-97
PubMed ID: 12534470