Pseudomonas aeruginosa PAO1, PA3162 (rpsA)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA3162
Name	rpsA
Replicon	chromosome
Genomic location	3548110 - 3549789 (- strand)
Transposon Mutants in orthologs	2 transposon mutants in orthologs

Cross-References

RefSeq	NP_251852.1
GI	15598358
Affymetrix	PA3162_rpsA_at
Entrez	882562
GenBank	AAG06550.1
INSDC	AAG06550.1
NCBI Locus Tag	PA3162
protein_id(GenBank)	gb|AAG06550.1|AE004740_3|gnl|PseudoCAP|PA3162
TIGR	NTL03PA03162
UniParc	UPI00000C5930
UniProtKB Acc	Q9HZ71
UniProtKB ID	RS1_PSEAE
UniRef100	UniRef100_Q9HZ71
UniRef50	UniRef50_P0AG69
UniRef90	UniRef90_Q9HZ71

Product

Feature Type	CDS
Coding Frame	1
Product Name	30S ribosomal protein S1 Product Name Confidence: Class 2
Synonyms
Evidence for Translation	Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602). Detected using LC-MS/MS (61.87 kDa, PMID:21751344) Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7)	-30.82
Kyte-Doolittle Hydrophobicity Value	-0.343
Molecular Weight (kDa)	61.9
Isoelectric Point (pI)	4.56

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Class 1 Outer Membrane Vesicle Class 1 Periplasmic Class 1 Cytoplasmic Class 3 20472543
Additional evidence for subcellular localization	Detected in a periplasmic protein preparation by spheroplasting by lysozyme and sucrose.(PMID:19333994)

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 598 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG002453 (535 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA3162 Search term: rpsA Search term: 30S ribosomal protein S1

Human Homologs

Ensembl 110, assembly GRCh38.p14

ENSP00000480986 zinc finger CCHC-type containing 17 [Source:HGNC Symbol;Acc:HGNC:30246] E-value: 1.9e-07 Percent Identity: 38.0

References

Ribosomal protein S1 specifically binds to the 5' untranslated region of the Pseudomonas aeruginosa stationary-phase sigma factor rpoS mRNA in the logarithmic phase of growth. Sevo M, Buratti E, Venturi V J Bacteriol 2004 Aug;186(15):4903-9 PubMed ID: 15262927

Primary structure of Escherichia coli ribosomal protein S1 and of its gene rpsA. Schnier J, Kimura M, Foulaki K, Subramanian AR, Isono K, Wittmann-Liebold B Proc Natl Acad Sci U S A 1982 Feb;79(4):1008-11 PubMed ID: 7041110

Ribosomal protein S1 is required for translation of most, if not all, natural mRNAs in Escherichia coli in vivo. Sørensen MA, Fricke J, Pedersen S J Mol Biol 1998 Jul 24;280(4):561-9 PubMed ID: 9677288

A probable mixed-function supraoperon in Pseudomonas exhibits gene organization features of both intergenomic conservation and gene shuffling. Xie G, Bonner CA, Jensen RA J Mol Evol 1999 Jul;49(1):108-21 PubMed ID: 10368439

Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen. Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM Proteomics 2009 Apr;9(7):1901-15 PubMed ID: 19333994

Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa. Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS Proteomics 2011 Aug;11(16):3424-9 PubMed ID: 21751344