Pseudomonas aeruginosa PAO1, PA3544 (algE)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
Locus Tag
Synonym: alg76
Replicon chromosome
Genomic location 3968449 - 3969921 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1


RefSeq NP_252234.1
GI 15598740
Affymetrix PA3544_algE_at
Entrez 879838
GenBank AAG06932.1
INSDC AAG06932.1
NCBI Locus Tag PA3544
protein_id(GenBank) gb|AAG06932.1|AE004775_3|gnl|PseudoCAP|PA3544
UniParc UPI000012584E
UniProtKB Acc P18895
UniRef100 UniRef100_P18895
UniRef50 UniRef50_P18895
UniRef90 UniRef90_P18895


Feature Type CDS
Coding Frame 1
Product Name
Alginate production outer membrane protein AlgE precursor
Product Name Confidence: Class 1
Synonyms alginate production protein AlgE
Evidence for Translation
Charge (pH 7) -20.33
Kyte-Doolittle Hydrophobicity Value -0.642
Molecular Weight (kDa) 54.4
Isoelectric Point (pI) 4.67

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Class 1
Outer Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4XNL TRANSPORT PROTEIN 01/15/15 X-ray structure of AlgE2 Pseudomonas aeruginosa PAO1 2.9 X-RAY DIFFRACTION 100.0
4XNK TRANSPORT PROTEIN 01/15/15 X-ray structure of AlgE1 Pseudomonas aeruginosa PAO1 2.8 X-RAY DIFFRACTION 100.0
4AZL MEMBRANE PROTEIN 06/26/12 In meso structure of alginate transporter, AlgE, from Pseudomoas aeruginosa, PAO1, crystal form 2. PSEUDOMONAS AERUGINOSA 2.8 X-RAY DIFFRACTION 100.0
3RBH TRANSPORT PROTEIN 03/29/11 Structure of alginate export protein AlgE from Pseudomonas aeruginosa Pseudomonas aeruginosa 2.301 X-RAY DIFFRACTION 100.0
5D5D TRANSPORT PROTEIN 08/10/15 In meso in situ serial X-ray crystallography structure of AlgE at 100 K Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) 2.4 X-RAY DIFFRACTION 100.0
7ACG MEMBRANE PROTEIN 09/10/20 In meso structure of the alginate exporter, AlgE, from Pseudomonas aeruginosa in 9.8 monoacylglycerol Pseudomonas aeruginosa 1.85 X-RAY DIFFRACTION 100.0
4B61 MEMBRANE PROTEIN 08/08/12 In meso structure of alginate transporter, AlgE, from Pseudomoas aeruginosa, PAO1. Crystal form 3. PSEUDOMONAS AERUGINOSA PAO1 2.402 X-RAY DIFFRACTION 100.0
4AFK MEMBRANE PROTEIN 01/19/12 In meso structure of alginate transporter, AlgE, from Pseudomonas aeruginosa, PAO1 PSEUDOMONAS AERUGINOSA 1.897 X-RAY DIFFRACTION 100.0
5IYU MEMBRANE PROTEIN 03/24/16 AlgE_CIM Pseudomonas aeruginosa PAO1 2.7 X-RAY DIFFRACTION 100.0

Virulence Evidence

Source 1
Database VFDB : VF0091
Category Alginate biosynthesis
Host Organism

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 6 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002122 (490 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA3544
Search term: algE

Human Homologs


Mucoid conversion of Pseudomonas aeruginosa by hydrogen peroxide: a mechanism for virulence activation in the cystic fibrosis lung.
Mathee K, Ciofu O, Sternberg C, Lindum PW, Campbell JIA, Jensen P, Johnsen AH, Givskov M, Ohman DE, Søren M, Høiby N, Kharazmi A
Microbiology (Reading) 1999 Jun;145 ( Pt 6):1349-1357
PubMed ID: 10411261
Nucleotide sequence and expression of the algE gene involved in alginate biosynthesis by Pseudomonas aeruginosa.
Chu L, May TB, Chakrabarty AM, Misra TK
Gene 1991 Oct 30;107(1):1-10
PubMed ID: 1743507