Pseudomonas aeruginosa UCBPP-PA14, PA14_32380 (oprN)

Gene Feature Overview

Strain	Pseudomonas aeruginosa UCBPP-PA14 (Lee et al., 2006) GCF_000014625.1|latest
Locus Tag	PA14_32380
Name	oprN
Replicon	chromosome
Genomic location	2812739 - 2814157 (- strand)
Transposon Mutants	1 transposon mutants in UCBPP-PA14
Transposon Mutants in orthologs	2 transposon mutants in orthologs

Cross-References

RefSeq	YP_790742.1
GI	116050439
Entrez	4382682
INSDC	ABJ11675.1
NCBI Locus Tag	PA14_32380
UniParc	UPI0000395DCB
UniProtKB Acc	Q02MU4
UniProtKB ID	Q02MU4_PSEAB
UniRef100	UniRef100_Q02MU4
UniRef50	UniRef50_Q88HD3
UniRef90	UniRef90_Q02MU4

Product

Feature Type	CDS
Coding Frame	1
Product Name	multidrug efflux outer membrane protein OprN precursor
Synonyms
Evidence for Translation
Charge (pH 7)	-6.52
Kyte-Doolittle Hydrophobicity Value	-0.293
Molecular Weight (kDa)	51.2
Isoelectric Point (pI)	5.08

Subcellular localization

Individual Mappings	Localization Confidence PMID Outer Membrane Class 3 20472543
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name	Definition	Accession	Model Type (?)	SNP	Cutoff	Bit Score	Percent Identity	CARD Version
OprN	OprN is the outer membrane channel component of the MexEF-OprN multidrug efflux complex. [PMID:10515918]	ARO:3000805	protein homolog model		Strict	876.3	99.79	3.2.5

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
5IUY	TRANSPORT PROTEIN	03/18/16	Structural insights of the outer-membrane channel OprN	Pseudomonas aeruginosa PAO1	2.29	X-RAY DIFFRACTION	100.0
5NSW	MEMBRANE PROTEIN	04/27/17	Xenon for tunnelling analysis of the efflux pump component OprN.	Pseudomonas aeruginosa PAO1	2.5	X-RAY DIFFRACTION	100.0
5AZO	MEMBRANE PROTEIN	10/21/15	Crystal structure of a membrane protein from Pseudomonas aeruginosa	Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)	2.7	X-RAY DIFFRACTION	100.0
5AZP	MEMBRANE PROTEIN	10/21/15	Crystal structure of a membrane protein from Pseudomonas aeruginosa	Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)	1.69	X-RAY DIFFRACTION	100.0

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 300 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG003062 (529 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA14_32380 Search term: oprN Search term: multidrug efflux outer membrane protein OprN precursor

Human Homologs

References