Pseudomonas aeruginosa UCBPP-PA14, PA14_51430 (pqsA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa UCBPP-PA14 (Lee et al., 2006)
GCF_000014625.1|latest
Locus Tag
PA14_51430
Name
pqsA
Replicon chromosome
Genomic location 4569916 - 4571469 (- strand)
Transposon Mutants 1 transposon mutants in UCBPP-PA14
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq YP_792275.1
GI 116048924
Entrez 4379980
INSDC ABJ10160.1
NCBI Locus Tag PA14_51430
UniParc UPI0000396581
UniProtKB Acc Q02IG1
UniProtKB ID Q02IG1_PSEAB
UniRef100 UniRef100_S0IZ18
UniRef50 UniRef50_Q9I4X3
UniRef90 UniRef90_Q9I4X3

Product

Feature Type CDS
Coding Frame 1
Product Name
PqsA
Product Name Confidence: Class 1
Synonyms coenzyme A ligase
Evidence for Translation
Charge (pH 7) -6.98
Kyte-Doolittle Hydrophobicity Value -0.054
Molecular Weight (kDa) 56.5
Isoelectric Point (pI) 6.15

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5OE3 LIGASE 07/07/17 Crystal structure of the N-terminal domain of PqsA in complex with anthraniloyl-AMP (crystal form 1) Pseudomonas aeruginosa PAO1 1.43 X-RAY DIFFRACTION 99.0
5OE6 LIGASE 07/07/17 Crystal structure of the N-terminal domain of PqsA in complex with 6-fluoroanthraniloyl-AMP (crystal form 1) Pseudomonas aeruginosa PAO1 1.67 X-RAY DIFFRACTION 99.0
5OE5 LIGASE 07/07/17 Crystal structure of the N-terminal domain of PqsA in complex with anthraniloyl-AMP (crystal form 3) Pseudomonas aeruginosa PAO1 1.74 X-RAY DIFFRACTION 99.0
5OE4 LIGASE 07/07/17 Crystal structure of the N-terminal domain of PqsA in complex with anthraniloyl-AMP (crystal form 2) Pseudomonas aeruginosa PAO1 1.904 X-RAY DIFFRACTION 99.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 599 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000959 (296 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA14_51430
Search term: pqsA
Search term: PqsA

Human Homologs

Ensembl 110, assembly GRCh38.p14
acyl-CoA synthetase short chain family member 1 [Source:HGNC Symbol;Acc:HGNC:16091]
E-value: 1.4e-25
Percent Identity: 24.7
Ensembl 110, assembly GRCh38.p14
acyl-CoA synthetase short chain family member 1 [Source:HGNC Symbol;Acc:HGNC:16091]
E-value: 1.4e-25
Percent Identity: 24.7

References

PqsA is required for the biosynthesis of 2,4-dihydroxyquinoline (DHQ), a newly identified metabolite produced by Pseudomonas aeruginosa and Burkholderia thailandensis.
Lépine F, Dekimpe V, Lesic B, Milot S, Lesimple A, Mamer OA, Rahme LG, Déziel E
Biol Chem 2007 Aug;388(8):839-45
PubMed ID: 17655503