Pseudomonas aeruginosa UCBPP-PA14, PA14_58420 (dppA4)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag
PA14_58420
Name
dppA4
Replicon chromosome
Genomic location 5203987 - 5205588 (+ strand)
Transposon Mutants 2 transposon mutants in UCBPP-PA14
Transposon Mutants in orthologs 5 transposon mutants in orthologs

Cross-References

RefSeq YP_792847.1
GI 116052533
Entrez 4382623
INSDC ABJ13769.1
NCBI Locus Tag PA14_58420
UniParc UPI00003963AF
UniProtKB Acc Q02GT9
UniProtKB ID Q02GT9_PSEAB
UniRef100 UniRef100_S0J4J5
UniRef50 UniRef50_Q87WI5
UniRef90 UniRef90_Q9HVR9

Product

Feature Type CDS
Coding Frame 1
Product Name
dipeptide ABC transporter substrate-binding protein DppA4
Product Name Confidence: Class 1
Evidence for Translation
Charge (pH 7) -2.49
Kyte-Doolittle Hydrophobicity Value -0.265
Molecular Weight (kDa) 58.8
Isoelectric Point (pI) 6.59

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 558 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004089 (529 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA14_58420
Search term: dppA4

Transposon Insertions in UCBPP-PA14

Type MAR2xT7 transposon (wild type background) insertion site
Mutant ID PA14NR: 44163
Genomic Position 5205538
Position in ORF 1552(1602)
Reference 16477005
Type MAR2xT7 transposon (wild type background) insertion site
Mutant ID PA14NR: 57090
Genomic Position 5204117
Position in ORF 131(1602)
Reference 16477005

Transposon Insertions in Orthologs

Type mini-Tn5 lux transposon insertion site (Transcriptional fusion)
Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag PA4502
Mutant ID UBC: PAO1_lux_20_C1
Genomic Position 5040621
Position in ORF 217(1596)
Reference 15805499
Type mini-Tn5 lux transposon insertion site (Transcriptional fusion)
Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag PA4502
Mutant ID UBC: PAO1_lux_20_E1
Genomic Position 5040619
Position in ORF 215(1596)
Reference 15805499
Type mini-Tn5 lux transposon insertion site (Transcriptional fusion)
Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag PA4502
Mutant ID UBC: PAO1_lux_24_E1
Genomic Position 5040619
Position in ORF 215(1596)
Reference 15805499
Type ISphoA/hah insertion site
Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag PA4502
Mutant ID UWGC: 33118
Genomic Position 5041910
Position in ORF 1506(1596)
Reference 14617778
Type ISphoA/hah insertion site
Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag PA4502
Mutant ID UWGC: 55965
Genomic Position 5041248
Position in ORF 844(1596)
Reference 14617778

References

High-throughput screening of dipeptide utilization mediated by the ABC transporter DppBCDF and its substrate-binding proteins DppA1-A5 in Pseudomonas aeruginosa.
Pletzer D(1), Lafon C(2), Braun Y(1), Köhler T(3), Page MG(4), Mourez M(2), Weingart H(1).
PLoS One. 2014 Oct 22;9(10):e111311.
PubMed ID: 25338022