Pseudomonas aeruginosa PAO1, PA0171 (siaB)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
Locus Tag
Replicon chromosome
Genomic location 194206 - 194748 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentInvolved in twitching motility
CommentSiaB is a functional Mg2+-dependent protein kinase with activity towards the non-phosphorylated threonine T68 of SiaC [PMID:33156827]
Comment The SiaABC threonine phosphorylation pathway controls aggregate/biofilm formation in response to carbon availability (glucose, succinate, ethanol, 2,3-butanediol) [PMID:33156827]


RefSeq NP_248861.1
GI 15595369
Affymetrix PA0171_at
Entrez 882156
GenBank AAG03561.1
INSDC AAG03561.1
NCBI Locus Tag PA0171
protein_id(GenBank) gb|AAG03561.1|AE004455_2|gnl|PseudoCAP|PA0171
UniParc UPI00000C4F7A
UniProtKB Acc Q9I6W1
UniRef100 UniRef100_W0YK48
UniRef50 UniRef50_A0A052I5K0
UniRef90 UniRef90_A6UXW2


Feature Type CDS
Coding Frame 1
Product Name
Product Name Confidence: Class 1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 0.13
Kyte-Doolittle Hydrophobicity Value -0.254
Molecular Weight (kDa) 19.8
Isoelectric Point (pI) 7.06

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6KKO GENE REGULATION 07/26/19 The crystal structure of SiaB-SiaC complex from Pseudomonas aeruginosa Pseudomonas aeruginosa 2.099 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 51 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000169 (326 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA0171
Search term: siaB
Search term: SiaB

Human Homologs


Identification of two new genes involved in twitching motility in Pseudomonas aeruginosa.
Shan Z, Xu H, Shi X, Yu Y, Yao H, Zhang X, Bai Y, Gao C, Saris PEJ, Qiao M
Microbiology (Reading) 2004 Aug;150(Pt 8):2653-2661
PubMed ID: 15289561
Autolysis and autoaggregation in Pseudomonas aeruginosa colony morphology mutants.
D'Argenio DA, Calfee MW, Rainey PB, Pesci EC
J Bacteriol 2002 Dec;184(23):6481-9
PubMed ID: 12426335
The SiaABC threonine phosphorylation pathway controls biofilm formation in response to carbon availability in Pseudomonas aeruginosa.
Poh WH, Lin J, Colley B, Müller N, Goh BC, Schleheck D, El Sahili A, Marquardt A, Liang Y, Kjelleberg S, Lescar J, Rice SA, Klebensberger J
PLoS One 2020;15(11):e0241019
PubMed ID: 33156827