Pseudomonas aeruginosa PAO1, PA0212 (mdcE)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0212
Name
mdcE
Replicon chromosome
Genomic location 240931 - 241737 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_248903.1
GI 15595409
Affymetrix PA0212_mdcE_at
Entrez 879507
GenBank AAG03601.1
INSDC AAG03601.1
NCBI Locus Tag PA0212
protein_id(GenBank) gb|AAG03601.1|AE004459_5|gnl|PseudoCAP|PA0212
TIGR NTL03PA00213
UniParc UPI00000C4F9B
UniProtKB Acc Q9I6S6
UniProtKB ID Q9I6S6_PSEAE
UniRef100 UniRef100_Q9I6S6
UniRef50 UniRef50_W0V3F6
UniRef90 UniRef90_Q9I6S6

Product

Feature Type CDS
Coding Frame 1
Product Name
malonate decarboxylase gamma subunit
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 0.17
Kyte-Doolittle Hydrophobicity Value 0.044
Molecular Weight (kDa) 28.5
Isoelectric Point (pI) 7.08

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5VIP TRANSFERASE 04/17/17 Crystal structure of Pseudomonas malonate decarboxylase MdcD-MdcE hetero-dimer Pseudomonas aeruginosa 1.857 X-RAY DIFFRACTION 100.0
5VIT TRANSFERASE 04/17/17 Crystal structure of a Pseudomonas malonate decarboxylase hetero-tetramer in complex with malonate Pseudomonas aeruginosa; Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1); Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5) 2.203 X-RAY DIFFRACTION 100.0
5VJ1 TRANSFERASE 04/18/17 Crystal structure of a Pseudomonas malonate decarboxylase hetero-tetramer in complex with coenzyme A Pseudomonas aeruginosa; Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1); Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5) 2.995 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 57 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000211 (478 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0212
Search term: mdcE

Human Homologs

References

Sequence of a gene cluster from Klebsiella pneumoniae encoding malonate decarboxylase and expression of the enzyme in Escherichia coli.
Hoenke S, Schmid M, Dimroth P
Eur J Biochem 1997 Jun 1;246(2):530-8
PubMed ID: 9208947