Pseudomonas aeruginosa PAO1, PA2998 (nqrB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2998
Name
nqrB
Replicon chromosome
Genomic location 3354947 - 3356158 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_251688.1
GI 15598194
Affymetrix PA2998_nqrB_at
Entrez 880205
GenBank AAG06386.1
INSDC AAG06386.1
NCBI Locus Tag PA2998
protein_id(GenBank) gb|AAG06386.1|AE004724_15|gnl|PseudoCAP|PA2998
TIGR NTL03PA02998
UniParc UPI000013043F
UniProtKB Acc Q9HZK7
UniProtKB ID NQRB_PSEAE
UniRef100 UniRef100_B7UZT8
UniRef50 UniRef50_Q56587
UniRef90 UniRef90_B7UZT8

Product

Feature Type CDS
Coding Frame 1
Product Name
Na+-translocating NADH:ubiquinone oxidoreductase subunit Nrq2
Product Name Confidence: Class 2
Synonyms Na+-translocating NADH:ubiquinone oxidoreductase beta subunit
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 1.09
Kyte-Doolittle Hydrophobicity Value 0.571
Molecular Weight (kDa) 44.1
Isoelectric Point (pI) 7.32

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database Victors : 4244
Category
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Rattus norvegicus
Notes
Infection model Rat agar bead model of chronic lung infection
Reference 14641575

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 230 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002583 (339 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2998
Search term: nqrB

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
NADH:ubiquinone oxidoreductase of Vibrio alginolyticus: purification, properties, and reconstitution of the Na+ pump.
Pfenninger-Li XD, Albracht SP, van Belzen R, Dimroth P
Biochemistry 1996 May 21;35(20):6233-42
PubMed ID: 8639563
Identification of six subunits constituting Na+-translocating NADH-quinone reductase from the marine Vibrio alginolyticus.
Nakayama Y, Hayashi M, Unemoto T
FEBS Lett 1998 Jan 30;422(2):240-2
PubMed ID: 9490015