Pseudomonas aeruginosa PAO1, PA3020

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA3020
Name	Synonym: slt
Replicon	chromosome
Genomic location	3381963 - 3383891 (+ strand)
Transposon Mutants	4 transposon mutants in PAO1
Transposon Mutants in orthologs	1 transposon mutants in orthologs

Cross-References

RefSeq	NP_251710.1
GI	15598216
Affymetrix	PA3020_at
Entrez	878768
GenBank	AAG06408.1
INSDC	AAG06408.1
NCBI Locus Tag	PA3020
protein_id(GenBank)	gb|AAG06408.1|AE004727_4|gnl|PseudoCAP|PA3020
TIGR	NTL03PA03020
UniParc	UPI00000C58CF
UniProtKB Acc	Q9HZI6
UniProtKB ID	Q9HZI6_PSEAE
UniRef100	UniRef100_Q9HZI6
UniRef50	UniRef50_L8MR62
UniRef90	UniRef90_A0A022P9S3

Product

Feature Type	CDS
Coding Frame	1
Product Name	probable soluble lytic transglycosylase Product Name Confidence: Class 3
Synonyms
Evidence for Translation	Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7)	19.05
Kyte-Doolittle Hydrophobicity Value	-0.411
Molecular Weight (kDa)	73.4
Isoelectric Point (pI)	9.82

Subcellular localization

Individual Mappings	Localization Confidence PMID Periplasmic Class 2 Periplasmic Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
6FBT	LYASE	12/19/17	The X-ray Structure of Lytic Transglycosylase Slt from Pseudomonas aeruginosa in complex with the reaction product NAG-anhNAMpentapeptide	Pseudomonas aeruginosa; SYNTHETIC CONSTRUCT	2.5	X-RAY DIFFRACTION	100.0
6FCU	LYASE	12/21/17	The X-ray Structure of Lytic Transglycosylase Slt inactive mutant E503Q from Pseudomonas aeruginosa in complex with 4(NAG-NAMpentapeptide)	Pseudomonas aeruginosa	3.2	X-RAY DIFFRACTION	99.8
6FCQ	LYASE	12/21/17	The X-ray Structure of Lytic Transglycosylase Slt inactive mutant E503Q from Pseudomonas aeruginosa in complex with Bulgecin A	Pseudomonas aeruginosa	3.1	X-RAY DIFFRACTION	99.8
6FCS	LYASE	12/21/17	The X-ray Structure of Lytic Transglycosylase Slt inactive mutant E503Q from Pseudomonas aeruginosa in complex with NAG-NAMpentapeptide-NAG-NAMpentapeptide	Pseudomonas aeruginosa	2.9	X-RAY DIFFRACTION	99.8
6FCR	LYASE	12/21/17	The X-ray Structure of Lytic Transglycosylase Slt inactive mutant E503Q from Pseudomonas aeruginosa in complex with NAG-NAMtetrapeptide-NAG-anhNAMtetrapeptide	Pseudomonas aeruginosa	2.75	X-RAY DIFFRACTION	99.8
6FC4	LYASE	12/20/17	The X-ray Structure of Lytic Transglycosylase Slt inactive mutant E503Q from Pseudomonas aeruginosa	Pseudomonas aeruginosa	3.05	X-RAY DIFFRACTION	99.8
5OHU	LYASE	07/18/17	The X-ray Structure of Lytic Transglycosylase Slt from Pseudomonas aeruginosa	Pseudomonas aeruginosa	2.2	X-RAY DIFFRACTION	99.8

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 399 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG002562 (535 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA3020 Search term: probable soluble lytic transglycosylase

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen. Lewenza S, Gardy JL, Brinkman FS, Hancock RE Genome Res 2005 Feb;15(2):321-9 PubMed ID: 15687295

Murein-metabolizing enzymes from Escherichia coli: sequence analysis and controlled overexpression of the slt gene, which encodes the soluble lytic transglycosylase. Engel H, Kazemier B, Keck W J Bacteriol 1991 Nov;173(21):6773-82 PubMed ID: 1938883