Pseudomonas aeruginosa PAO1, PA3082 (gbt)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3082
Name
gbt
Replicon chromosome
Genomic location 3457969 - 3459933 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 3 transposon mutants in orthologs
CommentLocalication to outer membrane vesicle confirmed in three separate studies (PMID:22909304, PMID:21751344 and PMID:26303878).

Cross-References

RefSeq NP_251772.1
GI 15598278
Affymetrix PA3082_at
Entrez 882950
GenBank AAG06470.1
INSDC AAG06470.1
NCBI Locus Tag PA3082
protein_id(GenBank) gb|AAG06470.1|AE004732_4|gnl|PseudoCAP|PA3082
TIGR NTL03PA03082
UniParc UPI00000C5909
UniProtKB Acc Q9HZC6
UniProtKB ID Q9HZC6_PSEAE
UniRef100 UniRef100_Q9HZC6
UniRef50 UniRef50_Q9HZC6
UniRef90 UniRef90_Q9HZC6

Product

Feature Type CDS
Coding Frame 1
Product Name
glycine betaine transmethylase
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Detected using LC-MS/MS (71.40 kDa, PMID:21751344)
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -22.31
Kyte-Doolittle Hydrophobicity Value -0.349
Molecular Weight (kDa) 71.4
Isoelectric Point (pI) 4.50

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Outer Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 45 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002510 (342 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3082
Search term: gbt

Human Homologs

References

Glycine betaine transmethylase mutant of Pseudomonas aeruginosa.
Serra AL, Mariscotti JF, Barra JL, Lucchesi GI, Domenech CE, Lisa AT
J Bacteriol 2002 Aug;184(15):4301-3
PubMed ID: 12107149
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
Identification of proteins associated with the Pseudomonas aeruginosa biofilm extracellular matrix.
Toyofuku M, Roschitzki B, Riedel K, Eberl L
J Proteome Res 2012 Oct 5;11(10):4906-15
PubMed ID: 22909304
Proteome Profiles of Outer Membrane Vesicles and Extracellular Matrix of Pseudomonas aeruginosa Biofilms.
Couto N, Schooling SR, Dutcher JR, Barber J
J Proteome Res 2015 Oct 2;14(10):4207-22
PubMed ID: 26303878