Pseudomonas aeruginosa PAO1, PA3225

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3225
Name
Replicon chromosome
Genomic location 3612559 - 3613488 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentPA3225 promotes antibiotic susceptibility by acting as a transcriptional repressor of the MexAB-OprM efflux pump as well as of putative antibiotic resistance determinants encoded by PA1210, PA2864, and PA3228.

Cross-References

RefSeq NP_251915.1
GI 15598421
Affymetrix PA3225_at
DNASU PaCD00007419
Entrez 882530
GenBank AAG06613.1
INSDC AAG06613.1
NCBI Locus Tag PA3225
protein_id(GenBank) gb|AAG06613.1|AE004745_10|gnl|PseudoCAP|PA3225
TIGR NTL03PA03225
UniParc UPI00000C595E
UniProtKB Acc Q9HZ15
UniProtKB ID Q9HZ15_PSEAE
UniRef100 UniRef100_W0YWR1
UniRef50 UniRef50_Q4KGG2
UniRef90 UniRef90_A6V2J7

Product

Feature Type CDS
Coding Frame 1
Product Name
transcriptional regulator
Product Name Confidence: Class 1
Synonyms probable transcriptional regulator
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 1.62
Kyte-Doolittle Hydrophobicity Value -0.160
Molecular Weight (kDa) 34.1
Isoelectric Point (pI) 7.44

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 402 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002388 (533 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3225

Human Homologs

References

PA3225 Is a Transcriptional Repressor of Antibiotic Resistance Mechanisms in Pseudomonas aeruginosa.
Hall CW, Zhang L, Mah TF
Antimicrob Agents Chemother 2017 Aug;61(8)
PubMed ID: 28584154