Pseudomonas aeruginosa PAO1, PA3244 (minD)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3244
Name
minD
Replicon chromosome
Genomic location 3631618 - 3632433 (+ strand)
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_251934.1
GI 15598440
Affymetrix PA3244_minD_at
DNASU PaCD00007430
Entrez 882407
GenBank AAG06632.1
INSDC AAG06632.1
NCBI Locus Tag PA3244
protein_id(GenBank) gb|AAG06632.1|AE004747_5|gnl|PseudoCAP|PA3244
TIGR NTL03PA03244
UniParc UPI00000C596E
UniProtKB Acc Q9HYZ6
UniProtKB ID Q9HYZ6_PSEAE
UniRef100 UniRef100_Q9HYZ6
UniRef50 UniRef50_P0AEZ5
UniRef90 UniRef90_Q9HYZ6

Product

Feature Type CDS
Coding Frame 1
Product Name
cell division inhibitor MinD
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Verified using two-dimensional gel electrophoresis, MALDI-TOF MS and peptide mass mapping(pI:5.6, molecular weight:29.6, PMID:21674802)
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -4.09
Kyte-Doolittle Hydrophobicity Value -0.107
Molecular Weight (kDa) 29.6
Isoelectric Point (pI) 5.49

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 2
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6RIQ PROTEIN FIBRIL 04/24/19 MinCD filament from Pseudomonas aeruginosa Pseudomonas aeruginosa 3.1 ELECTRON MICROSCOPY 99.6

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 532 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002364 (537 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3244
Search term: minD

Human Homologs

Ensembl 110, assembly GRCh38.p14
NUBP iron-sulfur cluster assembly factor 2, cytosolic [Source:HGNC Symbol;Acc:HGNC:8042]
E-value: 7.2e-05
Percent Identity: 23.9

References

The MinD protein is a membrane ATPase required for the correct placement of the Escherichia coli division site.
de Boer PA, Crossley RE, Hand AR, Rothfield LI
EMBO J 1991 Dec;10(13):4371-80
PubMed ID: 1836760
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Proteomics of the oxidative stress response induced by hydrogen peroxide and paraquat reveals a novel AhpC-like protein in Pseudomonas aeruginosa.
Hare NJ, Scott NE, Shin EH, Connolly AM, Larsen MR, Palmisano G, Cordwell SJ
Proteomics 2011 Aug;11(15):3056-69
PubMed ID: 21674802