Pseudomonas aeruginosa PAO1, PA3308 (hepA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3308
Name
hepA
Replicon chromosome
Genomic location 3707090 - 3709942 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_251998.1
GI 15598504
Affymetrix PA3308_hepA_at
Entrez 882473
GenBank AAG06696.1
NCBI Locus Tag PA3308
protein_id(GenBank) gb|AAG06696.1|AE004753_4|gnl|PseudoCAP|PA3308
TIGR NTL03PA03308
UniProtKB Acc Q9HYT6
UniProtKB ID RAPA_PSEAE

Product

Feature Type CDS
Coding Frame 1
Product Name
RNA helicase HepA
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -43.32
Kyte-Doolittle Hydrophobicity Value -0.309
Molecular Weight (kDa) 106.8
Isoelectric Point (pI) 4.84

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 317 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002310 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3308
Search term: hepA
Search term: RNA helicase HepA

Human Homologs

Ensembl 110, assembly GRCh38.p14
chromodomain helicase DNA binding protein 9 [Source:HGNC Symbol;Acc:HGNC:25701]
E-value: 1.0e-05
Percent Identity: 26.5
Ensembl 110, assembly GRCh38.p14
chromodomain helicase DNA binding protein 9 [Source:HGNC Symbol;Acc:HGNC:25701]
E-value: 1.0e-05
Percent Identity: 26.5
Ensembl 110, assembly GRCh38.p14
chromodomain helicase DNA binding protein 9 [Source:HGNC Symbol;Acc:HGNC:25701]
E-value: 1.0e-05
Percent Identity: 26.5
Ensembl 110, assembly GRCh38.p14
chromodomain helicase DNA binding protein 9 [Source:HGNC Symbol;Acc:HGNC:25701]
E-value: 1.0e-05
Percent Identity: 26.5
Ensembl 110, assembly GRCh38.p14
chromodomain helicase DNA binding protein 9 [Source:HGNC Symbol;Acc:HGNC:25701]
E-value: 1.0e-05
Percent Identity: 26.5

References

An expanding family of helicases within the 'DEAD/H' superfamily.
Bork P, Koonin EV
Nucleic Acids Res 1993 Feb 11;21(3):751-2
PubMed ID: 8382805
Disruption of Escherichia coli hepA, an RNA polymerase-associated protein, causes UV sensitivity.
Muzzin O, Campbell EA, Xia L, Severinova E, Darst SA, Severinov K
J Biol Chem 1998 Jun 12;273(24):15157-61
PubMed ID: 9614128
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994