Pseudomonas aeruginosa PAO1, PA3313

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA3313
Name
Replicon	chromosome
Genomic location	3715804 - 3716811 (+ strand)
Transposon Mutants	2 transposon mutants in PAO1
Transposon Mutants in orthologs	3 transposon mutants in orthologs
Comment	Structural similarity to 5LQ1 (phosphonate Binding Protein PtxB from Trichodesmium erythraeum, root-mean-square deviation:0.8 angstroms). Sequence similarity to 5LQ5 (75%/39,25%) (PO3). Final predicted ligand: organophosphorus compounds (PMID: 31631799)

Cross-References

RefSeq	NP_252003.1
GI	15598509
Affymetrix	PA3313_at
DNASU	PaCD00007458
Entrez	882478
GenBank	AAG06701.1
INSDC	AAG06701.1
NCBI Locus Tag	PA3313
protein_id(GenBank)	gb|AAG06701.1|AE004753_9|gnl|PseudoCAP|PA3313
TIGR	NTL03PA03313
UniParc	UPI00000C59AA
UniProtKB Acc	Q9HYT1
UniProtKB ID	Q9HYT1_PSEAE
UniRef100	UniRef100_Q9HYT1
UniRef50	UniRef50_I7CB62
UniRef90	UniRef90_Q9HYT1

Product

Feature Type	CDS
Coding Frame	1
Product Name	putative periplasmic organophosphorous compound-binding protein Product Name Confidence: Class 3
Synonyms
Evidence for Translation	Detected by iTRAQ (PMID:21863142). Detected by two-dimensional gel electrophoresis and protein mass mapping(PMID:21863142). Detected by MALDI-TOF/TOF (PMID:19333994). Detected by two-dimensional gel electrophoresis (PMID:21863142). Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7)	11.96
Kyte-Doolittle Hydrophobicity Value	-0.201
Molecular Weight (kDa)	36.5
Isoelectric Point (pI)	10.19

Subcellular localization

Individual Mappings	Localization Confidence PMID Periplasmic Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 253 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG002245 (807 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA3313 Search term: putative periplasmic organophosphorous compound-binding protein

Human Homologs

References

Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen. Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM Proteomics 2009 Apr;9(7):1901-15 PubMed ID: 19333994

Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient. Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB Front Microbiol 2011;2:162 PubMed ID: 21863142

Uncovering the structure and function of Pseudomonas aeruginosa periplasmic proteins by an in silico approach. Caprari S, Brandi V, Pasquadibisceglie A, Polticelli F J Biomol Struct Dyn 2020 Sep;38(15):4508-4520 PubMed ID: 31631799