Pseudomonas aeruginosa PAO1, PA3353 (flgZ)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3353
Name
flgZ
Replicon chromosome
Genomic location 3763723 - 3764472 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentN_terminal protein sequencing by Edman degradation revealed the amino acid sequence PNPFVEEAGP as the beginning of the FlgZ protein (PMID:30864240)

Cross-References

RefSeq NP_252043.1
GI 15598549
Affymetrix PA3353_at
DNASU PaCD00007470
Entrez 882520
GenBank AAG06741.1
INSDC AAG06741.1
NCBI Locus Tag PA3353
protein_id(GenBank) gb|AAG06741.1|AE004757_10|gnl|PseudoCAP|PA3353
TIGR NTL03PA03353
UniParc UPI00000C59CC
UniProtKB Acc Q9HYP3
UniProtKB ID Q9HYP3_PSEAE
UniRef100 UniRef100_Q9HYP3
UniRef50 UniRef50_Q88EQ6
UniRef90 UniRef90_U8NII6

Product

Feature Type CDS
Coding Frame 1
Product Name
FlgZ
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -4.85
Kyte-Doolittle Hydrophobicity Value -0.401
Molecular Weight (kDa) 28.3
Isoelectric Point (pI) 5.44

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 18 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002275 (534 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3353
Search term: flgZ
Search term: FlgZ

Human Homologs

References

PilZ Domain Protein FlgZ Mediates Cyclic Di-GMP-Dependent Swarming Motility Control in Pseudomonas aeruginosa.
Baker AE, Diepold A, Kuchma SL, Scott JE, Ha DG, Orazi G, Armitage JP, O'Toole GA
J Bacteriol 2016 Jul 1;198(13):1837-46
PubMed ID: 27114465
AmrZ Regulates Swarming Motility Through Cyclic di-GMP-Dependent Motility Inhibition and Controlling Pel Polysaccharide Production in Pseudomonas aeruginosa PA14.
Hou L, Debru A, Chen Q, Bao Q, Li K
Front Microbiol 2019;10:1847
PubMed ID: 31474950
Spatiotemporal control of FlgZ activity impacts Pseudomonas aeruginosa flagellar motility.
Bense S, Bruchmann S, Steffen A, Stradal TEB, Häussler S, Düvel J
Mol Microbiol 2019 Jun;111(6):1544-1557
PubMed ID: 30864240