Pseudomonas aeruginosa PAO1, PA3418 (ldh)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3418
Name
ldh
Replicon chromosome
Genomic location 3824748 - 3825773 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_252108.1
GI 15598614
Affymetrix PA3418_ldh_at
DNASU PaCD00007502
Entrez 879877
GenBank AAG06806.1
INSDC AAG06806.1
NCBI Locus Tag PA3418
protein_id(GenBank) gb|AAG06806.1|AE004763_1|gnl|PseudoCAP|PA3418
TIGR NTL03PA03418
UniParc UPI00000C5A00
UniProtKB Acc Q9HYI7
UniProtKB ID Q9HYI7_PSEAE
UniRef100 UniRef100_Q9HYI7
UniRef50 UniRef50_S6AZW7
UniRef90 UniRef90_Q9HYI7

Product

Feature Type CDS
Coding Frame 1
Product Name
leucine dehydrogenase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -7.30
Kyte-Doolittle Hydrophobicity Value 0.059
Molecular Weight (kDa) 35.6
Isoelectric Point (pI) 5.91

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
7VID OXIDOREDUCTASE 09/26/21 The crystal structure of L-leucine dehydrogenase from Pseudomonas aeruginosa Pseudomonas aeruginosa PAO1 2.5 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 344 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002212 (502 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3418
Search term: ldh

Human Homologs

References

Gene cloning and sequence determination of leucine dehydrogenase from Bacillus stearothermophilus and structural comparison with other NAD(P)+-dependent dehydrogenases.
Nagata S, Tanizawa K, Esaki N, Sakamoto Y, Ohshima T, Tanaka H, Soda K
Biochemistry 1988 Dec 13;27(25):9056-62
PubMed ID: 3069133
Leucine dehydrogenase from Bacillus stearothermophilus: identification of active-site lysine by modification with pyridoxal phosphate.
Matsuyama T, Soda K, Fukui T, Tanizawa K
J Biochem 1992 Aug;112(2):258-65
PubMed ID: 1400267