Pseudomonas aeruginosa PAO1, PA4007 (proA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4007
Name
proA
Synonym: proB
Replicon chromosome
Genomic location 4486847 - 4488112 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 3 transposon mutants in orthologs

Cross-References

RefSeq NP_252696.1
GI 15599202
Affymetrix PA4007_proA_at
Entrez 878970
GenBank AAG07394.1
INSDC AAG07394.1
NCBI Locus Tag PA4007
protein_id(GenBank) gb|AAG07394.1|AE004817_18|gnl|PseudoCAP|PA4007
TIGR NTL03PA04007
UniParc UPI00001322B6
UniProtKB Acc Q9HX20
UniProtKB ID PROA_PSEAE
UniRef100 UniRef100_Q9HX20
UniRef50 UniRef50_Q3SG61
UniRef90 UniRef90_Q9HX20

Product

Feature Type CDS
Coding Frame 1
Product Name
gamma-glutamyl phosphate reductase
Product Name Confidence: Class 2
Synonyms Glutamate-semialdehyde dehydrogenase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -9.94
Kyte-Doolittle Hydrophobicity Value -0.015
Molecular Weight (kDa) 45.0
Isoelectric Point (pI) 5.20

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 515 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001676 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4007
Search term: proA

Human Homologs

Ensembl 110, assembly GRCh38.p14
aldehyde dehydrogenase 18 family member A1 [Source:HGNC Symbol;Acc:HGNC:9722]
E-value: 1.3e-69
Percent Identity: 39.1
Ensembl 110, assembly GRCh38.p14
aldehyde dehydrogenase 18 family member A1 [Source:HGNC Symbol;Acc:HGNC:9722]
E-value: 1.3e-69
Percent Identity: 39.1

References

Proline biosynthesis in Escherichia coli. Purification and characterisation of glutamate-semialdehyde dehydrogenase.
Hayzer DJ, Leisinger T
Eur J Biochem 1982 Jan;121(3):561-5
PubMed ID: 7035170
Analysis of the Escherichia coli proBA locus by DNA and protein sequencing.
Deutch AH, Rushlow KE, Smith CJ
Nucleic Acids Res 1984 Aug 10;12(15):6337-55
PubMed ID: 6089111
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994