Pseudomonas aeruginosa PAO1, PA4225 (pchF)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4225
Name
pchF
Replicon chromosome
Genomic location 4731369 - 4736798 (- strand)
Transposon Mutants 7 transposon mutants in PAO1
Transposon Mutants in orthologs 10 transposon mutants in orthologs

Cross-References

RefSeq NP_252915.1
GI 15599421
Affymetrix PA4225_pchF_at
Entrez 880083
GenBank AAG07613.1
INSDC AAG07613.1
NCBI Locus Tag PA4225
protein_id(GenBank) gb|AAG07613.1|AE004839_6|gnl|PseudoCAP|PA4225
TIGR NTL03PA04226
UniParc UPI00000C5C7A
UniProtKB Acc Q9HWG4
UniProtKB ID Q9HWG4_PSEAE
UniRef100 UniRef100_Q9HWG4
UniRef50 UniRef50_Q399S4
UniRef90 UniRef90_Q9HWG4

Product

Feature Type CDS
Coding Frame 1
Product Name
pyochelin synthetase
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -27.50
Kyte-Doolittle Hydrophobicity Value -0.112
Molecular Weight (kDa) 197.1
Isoelectric Point (pI) 5.87

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown (This protein may have multiple localization sites) Class 3
Unknown (This protein may have multiple localization sites) Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database VFDB : VF0095
Category Pyochelin
Evidence
Host Organism
Notes
Reference

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 549 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001483 (2117 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4225
Search term: pchF

Human Homologs

Ensembl 110, assembly GRCh38.p14
acyl-CoA synthetase long chain family member 4 [Source:HGNC Symbol;Acc:HGNC:3571]
E-value: 6.1e-15
Percent Identity: 25.2

References

Dihydroaeruginoic acid synthetase and pyochelin synthetase, products of the pchEF genes, are induced by extracellular pyochelin in Pseudomonas aeruginosa.
Reimmann C, Serino L, Beyeler M, Haa D
Microbiology (Reading) 1998 Nov;144 ( Pt 11):3135-3148
PubMed ID: 9846750