Pseudomonas aeruginosa PAO1, PA4254 (rpsQ)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA4254
Name	rpsQ
Replicon	chromosome
Genomic location	4762659 - 4762925 (- strand)

Cross-References

RefSeq	NP_252944.1
GI	15599450
Affymetrix	PA4254_rpsQ_at
DNASU	PaCD00007848
Entrez	881770
GenBank	AAG07642.1
INSDC	AAG07642.1
NCBI Locus Tag	PA4254
protein_id(GenBank)	gb|AAG07642.1|AE004841_20|gnl|PseudoCAP|PA4254
TIGR	NTL03PA04255
UniParc	UPI00000C5C88
UniProtKB Acc	Q9HWE4
UniProtKB ID	RS17_PSEAE
UniRef100	UniRef100_A6UZJ7
UniRef50	UniRef50_Q5SHP7
UniRef90	UniRef90_A6UZJ7

Product

Feature Type	CDS
Coding Frame	1
Product Name	30S ribosomal protein S17 Product Name Confidence: Class 2
Synonyms
Evidence for Translation	Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7)	7.67
Kyte-Doolittle Hydrophobicity Value	-0.440
Molecular Weight (kDa)	10.1
Isoelectric Point (pI)	10.58

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
7UNW	RIBOSOME	04/11/22	Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-B)	Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT	2.6	ELECTRON MICROSCOPY	100.0
7UNV	RIBOSOME	04/11/22	Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-A)	Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT	2.7	ELECTRON MICROSCOPY	100.0
6SPF	RIBOSOME	09/01/19	Pseudomonas aeruginosa 70s ribosome from an aminoglycoside resistant clinical isolate	Pseudomonas aeruginosa	2.89	ELECTRON MICROSCOPY	100.0
7UNR	RIBOSOME	04/11/22	Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-A)	Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT	2.9	ELECTRON MICROSCOPY	100.0
6SPG	RIBOSOME	09/01/19	Pseudomonas aeruginosa 70s ribosome from a clinical isolate	Pseudomonas aeruginosa	3.34	ELECTRON MICROSCOPY	100.0
7UNU	RIBOSOME	04/11/22	Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-B)	Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT	2.9	ELECTRON MICROSCOPY	100.0
6SPC	RIBOSOME	09/01/19	Pseudomonas aeruginosa 30s ribosome from an aminoglycoside resistant clinical isolate	Pseudomonas aeruginosa	2.95	ELECTRON MICROSCOPY	100.0
6SPE	RIBOSOME	09/01/19	Pseudomonas aeruginosa 30s ribosome from a clinical isolate	Pseudomonas aeruginosa	3.6	ELECTRON MICROSCOPY	100.0

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 644 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG001456 (535 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA4254 Search term: rpsQ Search term: 30S ribosomal protein S17

Human Homologs

References

DNA sequences of promoter regions for the str and spc ribosomal protein operons in E. coli. Post LE, Arfsten AE, Reusser F, Nomura M Cell 1978 Sep;15(1):215-29 PubMed ID: 151587

A missense mutation in the gene coding for ribosomal protein S17 (rpsQ) leading to ribosomal assembly defectivity in Escherichia coli. Herzog A, Yaguchi M, Cabezón T, Corchuelo MC, Petre J, Bollen A Mol Gen Genet 1979 Mar 9;171(1):15-22 PubMed ID: 108517

Structure of the Escherichia coli S10 ribosomal protein operon. Zurawski G, Zurawski SM Nucleic Acids Res 1985 Jun 25;13(12):4521-6 PubMed ID: 3892488