Pseudomonas aeruginosa PAO1, PA4258 (rplV)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4258
Name
rplV
Replicon chromosome
Genomic location 4764243 - 4764575 (- strand)

Cross-References

RefSeq NP_252948.1
GI 15599454
Affymetrix PA4258_rplV_at
Entrez 881762
GenBank AAG07646.1
INSDC AAG07646.1
NCBI Locus Tag PA4258
protein_id(GenBank) gb|AAG07646.1|AE004841_24|gnl|PseudoCAP|PA4258
TIGR NTL03PA04259
UniParc UPI000000EB39
UniProtKB Acc Q9HWE0
UniProtKB ID RL22_PSEAE
UniRef100 UniRef100_A6UZJ3
UniRef50 UniRef50_B2HZL7
UniRef90 UniRef90_A4VHN5

Product

Feature Type CDS
Coding Frame 1
Product Name
50S ribosomal protein L22
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 10.44
Kyte-Doolittle Hydrophobicity Value -0.251
Molecular Weight (kDa) 11.9
Isoelectric Point (pI) 10.90

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
7UNW RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-B) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.6 ELECTRON MICROSCOPY 100.0
7UNV RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-A) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.7 ELECTRON MICROSCOPY 100.0
6SPD RIBOSOME 09/01/19 Pseudomonas aeruginosa 50s ribosome from a clinical isolate Pseudomonas aeruginosa 3.28 ELECTRON MICROSCOPY 100.0
7UNU RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-B) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.9 ELECTRON MICROSCOPY 100.0
6SPB RIBOSOME 09/01/19 Pseudomonas aeruginosa 50s ribosome from a clinical isolate with a mutation in uL6 Pseudomonas aeruginosa 2.82 ELECTRON MICROSCOPY 100.0
6SPG RIBOSOME 09/01/19 Pseudomonas aeruginosa 70s ribosome from a clinical isolate Pseudomonas aeruginosa 3.34 ELECTRON MICROSCOPY 100.0
7UNR RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-A) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.9 ELECTRON MICROSCOPY 100.0
6SPF RIBOSOME 09/01/19 Pseudomonas aeruginosa 70s ribosome from an aminoglycoside resistant clinical isolate Pseudomonas aeruginosa 2.89 ELECTRON MICROSCOPY 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 612 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001452 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4258
Search term: rplV

Human Homologs

References

Amino acid sequence of protein L22 from the large subunit of the Escherichia coli ribosome.
Wittmann-Liebold B, Greuer B
FEBS Lett 1980 Nov 17;121(1):105-12
PubMed ID: 7007072
Structure of the Escherichia coli S10 ribosomal protein operon.
Zurawski G, Zurawski SM
Nucleic Acids Res 1985 Jun 25;13(12):4521-6
PubMed ID: 3892488
A temperature-sensitive mutant of Escherichia coli with an alteration in ribosomal protein L22.
Burnette-Vick B, Champney WS, Musich PR
Genetica 1994;94(1):17-25
PubMed ID: 7729693