Pseudomonas aeruginosa PAO1, PA4266 (fusA1)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4266
Name
fusA1
Replicon chromosome
Genomic location 4769035 - 4771155 (- strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_252956.1
GI 15599462
Affymetrix PA4266_fusA1_at
Entrez 881744
GenBank AAG07654.1
INSDC AAG07654.1
NCBI Locus Tag PA4266
protein_id(GenBank) gb|AAG07654.1|AE004842_3|gnl|PseudoCAP|PA4266
TIGR NTL03PA04267
UniParc UPI0000129C9F
UniProtKB Acc Q9HWD2
UniProtKB ID EFG1_PSEAE
UniRef100 UniRef100_Q9HWD2
UniRef50 UniRef50_P43925
UniRef90 UniRef90_Q9HWD2

Product

Feature Type CDS
Coding Frame 1
Product Name
elongation factor G
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (77.78 kDa, PMID:21751344)
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -23.71
Kyte-Doolittle Hydrophobicity Value -0.304
Molecular Weight (kDa) 77.8
Isoelectric Point (pI) 4.80

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 2
Cytoplasmic Class 3
Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4FN5 TRANSLATION/ANTIBIOTIC 06/19/12 ELONGATION FACTOR G 1 (PSEUDOMONAS AERUGINOSA) IN COMPLEX WITH Argyrin B Actinoplanes sp.; Pseudomonas aeruginosa 2.9 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 669 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001445 (883 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4266
Search term: fusA1

Human Homologs

Ensembl 110, assembly GRCh38.p14
G elongation factor mitochondrial 1 [Source:HGNC Symbol;Acc:HGNC:13780]
E-value: 2.5e-150
Percent Identity: 42.1

References

The nucleotide sequence of the cloned tufA gene of Escherichia coli.
Yokota T, Sugisaki H, Takanami M, Kaziro Y
Gene 1980 Dec;12(1-2):25-31
PubMed ID: 7011903
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344