Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA4406
|
Name |
lpxC
Synonym: envA Synonym: asmB |
Replicon | chromosome |
Genomic location | 4938276 - 4939187 (- strand) |
Transposon Mutants in orthologs | 1 transposon mutants in orthologs |
RefSeq | NP_253096.1 |
GI | 15599602 |
Affymetrix | PA4406_lpxC_at |
DNASU | PaCD00007909 |
Entrez | 881292 |
GenBank | AAG07794.1 |
INSDC | AAG07794.1 |
NCBI Locus Tag | PA4406 |
protein_id(GenBank) | gb|AAG07794.1|AE004856_5|gnl|PseudoCAP|PA4406 |
TIGR | NTL03PA04407 |
UniParc | UPI000012E8E2 |
UniProtKB Acc | P47205 |
UniProtKB ID | LPXC_PSEAE |
UniRef100 | UniRef100_B7UZI4 |
UniRef50 | UniRef50_Q6D0I9 |
UniRef90 | UniRef90_Q87WZ1 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
UDP-3-O-acyl-N-acetylglucosamine deacetylase
|
Synonyms |
EnvA protein |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
|
Charge (pH 7) | -7.82 |
Kyte-Doolittle Hydrophobicity Value | -0.066 |
Molecular Weight (kDa) | 33.4 |
Isoelectric Point (pI) | 5.00 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
6MOD | HYDROLASE/INHIBITOR | 10/04/18 | Co-Crystal structure of P. aeruginosa LpxC-50432 complex | Pseudomonas aeruginosa | 1.85 | X-RAY DIFFRACTION | 99.7 |
7K9A | HYDROLASE | 09/29/20 | Crystal Structure of P. aeruginosa LpxC with N-Hydroxyformamide inhibitor | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) | 2 | X-RAY DIFFRACTION | 100.0 |
5VWM | Hydrolase/Hydrolase Inhibitor | 05/22/17 | Crystal structure of UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (LpxC) from Pseudomonas aeruginosa in complex with CHIR-090 inhibitor | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) | 1.8 | X-RAY DIFFRACTION | 100.0 |
5N8C | HYDROLASE | 02/23/17 | Crystal structure of Pseudomonas aeruginosa LpxC complexed with inhibitor | Pseudomonas aeruginosa | 1.9 | X-RAY DIFFRACTION | 99.7 |
6DUI | hydrolase/hydrolase inhibitor | 06/20/18 | Crystal structure of LpxC from Pseudomonas aeruginosa in complex with PT801 | Pseudomonas aeruginosa (strain LESB58) | 1.55 | X-RAY DIFFRACTION | 100.0 |
4LCG | HYDROLASE/ANTIBIOTIC | 06/21/13 | Crystal structure of the Pseudomonas aeruginosa LPXC/LPC-050 complex | Pseudomonas aeruginosa | 1.568 | X-RAY DIFFRACTION | 99.7 |
5DRR | hydrolase/hydrolase inhibitor | 09/16/15 | Crystal structure of the Pseudomonas aeruginosa LpxC/LPC-058 complex | Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) | 1.59 | X-RAY DIFFRACTION | 99.7 |
6E54 | hydrolase/hydrolase inhibitor | 07/19/18 | Crystal structure of LpxC from Pseudomonas aeruginosa in complex with ligand PT802 | Pseudomonas aeruginosa | 1.65 | X-RAY DIFFRACTION | 100.0 |
8E4A | BIOSYNTHETIC PROTEIN | 08/17/22 | Pseudomonas LpxC in complex with LPC-233 | Pseudomonas aeruginosa | 2.034 | X-RAY DIFFRACTION | 99.7 |
4FW7 | HYDROLASE/HYDROLASE INHIBITOR | 06/30/12 | Crystal Structure of the LpxC in complex with N-[(2S,3R)-3-HYDROXY-1-(HYDROXYAMINO)-1-OXOBUTAN-2-YL]BIPHENYL-4-CARBOXAMIDE inhibitor | Pseudomonas aeruginosa | 1.7 | X-RAY DIFFRACTION | 99.7 |
7K99 | HYDROLASE | 09/28/20 | Crystal Structure of P. aeruginosa LpxC with N-Hydroxyformamide inhibitor 19 | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) | 1.9 | X-RAY DIFFRACTION | 100.0 |
6MO5 | HYDROLASE/INHIBITOR | 10/04/18 | Co-Crystal structure of P. aeruginosa LpxC-50228 complex | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) | 1.851 | X-RAY DIFFRACTION | 99.7 |
6C9C | hydrolase/hydrolase inhibitor | 01/26/18 | Crystal structure of LpxC from Pseudomonas aeruginosa in complex with racemic ligand PT803 | Pseudomonas aeruginosa (strain UCBPP-PA14) | 2 | X-RAY DIFFRACTION | 100.0 |
5UPG | HYDROLASE | 02/02/17 | Crystal structure of LpxC from Pseudomonas aeruginosa in complex with PF-5081090 | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) | 1.7 | X-RAY DIFFRACTION | 100.0 |
6CAX | hydrolase/hydrolase inhibitor | 02/01/18 | Crystal structure of LpxC from Pseudomonas aeruginosa in complex with PT805 | Pseudomonas aeruginosa | 1.25 | X-RAY DIFFRACTION | 100.0 |
6MAE | HYDROLASE | 08/27/18 | CHAIN A. UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase PA-LPXC Complexed with (R)-3-((S)-3-(4-(cyclopropylethynyl)phenyl)-2-oxooxazolidin-5-yl)-N-hydroxy-2-methyl-2-(methylsulfonyl)propenamide | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) | 1.8 | X-RAY DIFFRACTION | 100.0 |
7CID | HYDROLASE | 07/07/20 | Crystal structure of P.aeruginosa LpxC in complex with inhibitor | Pseudomonas aeruginosa PAO1 | 2.49 | X-RAY DIFFRACTION | 99.7 |
5U3B | HYDROLASE | 12/01/16 | Pseudomonas aeruginosa LpxC in complex with NVS-LPXC-01 | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) | 2 | X-RAY DIFFRACTION | 100.0 |
4LCF | HYDROLASE/ANTIBIOTIC | 06/21/13 | Crystal structure of the Pseudomonas aeruginosa LPXC/LPC-014 complex | Pseudomonas aeruginosa | 1.599 | X-RAY DIFFRACTION | 99.7 |
6MO4 | HYDROLASE/INHIBITOR | 10/04/18 | Co-Crystal structure of P. aeruginosa LpxC-50067 complex | Pseudomonas aeruginosa PAO1 | 1.844 | X-RAY DIFFRACTION | 100.0 |
Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.
Drug Name | Source Accession | Source DB | Version | Target Accession | Target Description | Percent Identity | Alignment Length | E-Value |
(2R)-N-hydroxy-3-naphthalen-2-yl-2-[(naphthalen-2-ylsulfonyl)amino]propanamide | DB07861 | DrugBank | 5.1.4 | P47205 | UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase | 100.0 | 303 | 8.5e-167 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 387 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG003996 (535 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA4406
Search term: lpxC
Search term: UDP-3-O-acyl-N-acetylglucosamine deacetylase
|
Sequence analysis, transcriptional organization, and insertional mutagenesis of the envA gene of Escherichia coli.
Beall B, Lutkenhaus J
J Bacteriol 1987 Dec;169(12):5408-15
PubMed ID: 2824434
|
The envA permeability/cell division gene of Escherichia coli encodes the second enzyme of lipid A biosynthesis. UDP-3-O-(R-3-hydroxymyristoyl)-N-acetylglucosamine deacetylase.
Young K, Silver LL, Bramhill D, Cameron P, Eveland SS, Raetz CR, Hyland SA, Anderson MS
J Biol Chem 1995 Dec 22;270(51):30384-91
PubMed ID: 8530464
|
asmB, a suppressor locus for assembly-defective OmpF mutants of Escherichia coli, is allelic to envA (lpxC).
Kloser AW, Laird MW, Misra R
J Bacteriol 1996 Sep;178(17):5138-43
PubMed ID: 8752330
|
Cloning, expression, and purification of UDP-3-O-acyl-GlcNAc deacetylase from Pseudomonas aeruginosa: a metalloamidase of the lipid A biosynthesis pathway.
Hyland SA, Eveland SS, Anderson MS
J Bacteriol 1997 Mar;179(6):2029-37
PubMed ID: 9068651
|
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
|