Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA4453
|
Name |
|
Replicon | chromosome |
Genomic location | 4986152 - 4986799 (- strand) |
Transposon Mutants | 4 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 1 transposon mutants in orthologs |
Comment | Structural similarity to 2QGU: phospholipid binding protein from Ralstonia Solanacearum (root-mean-square deviation: 1.1 angstroms) |
RefSeq | NP_253143.1 |
GI | 15599649 |
Affymetrix | PA4453_at |
DNASU | PaCD00007929 |
Entrez | 880994 |
GenBank | AAG07841.1 |
INSDC | AAG07841.1 |
NCBI Locus Tag | PA4453 |
protein_id(GenBank) | gb|AAG07841.1|AE004859_10|gnl|PseudoCAP|PA4453 |
TIGR | NTL03PA04454 |
UniParc | UPI00000C5D18 |
UniProtKB Acc | Q9HVW4 |
UniProtKB ID | Q9HVW4_PSEAE |
UniRef100 | UniRef100_W0YYW6 |
UniRef50 | UniRef50_A0A080V9N0 |
UniRef90 | UniRef90_A0A080V9N0 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
putative phospholipid binding protein
|
Synonyms | |
Evidence for Translation |
Detected by MALDI-TOF/TOF (PMID:19333994).
Detected by iTRAQ (PMID:21863142).
Detected by two-dimensional gel electrophoresis and mass spectral analysis(PMID:21863142).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by two-dimensional gel electrophoresis (PMID:21863142).
|
Charge (pH 7) | 3.99 |
Kyte-Doolittle Hydrophobicity Value | -0.258 |
Molecular Weight (kDa) | 23.8 |
Isoelectric Point (pI) | 9.66 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
6HSY | LIPID TRANSPORT | 10/02/18 | Two-phospholipid-bound crystal structure of the substrate-binding protein Ttg2D from Pseudomonas aeruginosa | Pseudomonas aeruginosa PAO1 | 2.53 | X-RAY DIFFRACTION | 100.0 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 128 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG004042 (536 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA4453
Search term: putative phospholipid binding protein
|
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
|
Isolation and characterization of toluene-sensitive mutants from the toluene-resistant bacterium Pseudomonas putida GM73.
Kim K, Lee S, Lee K, Lim D
J Bacteriol 1998 Jul;180(14):3692-6
PubMed ID: 9658016
|
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
|
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142
|
Uncovering the structure and function of Pseudomonas aeruginosa periplasmic proteins by an in silico approach.
Caprari S, Brandi V, Pasquadibisceglie A, Polticelli F
J Biomol Struct Dyn 2020 Sep;38(15):4508-4520
PubMed ID: 31631799
|