Pseudomonas aeruginosa PAO1, PA4546 (pilS)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4546
Name
pilS
Replicon chromosome
Genomic location 5093378 - 5094970 (+ strand)
Transposon Mutants 6 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_253236.1
GI 15599742
Affymetrix PA4546_pilS_at
Entrez 877621
GenBank AAG07934.1
INSDC AAG07934.1
NCBI Locus Tag PA4546
protein_id(GenBank) gb|AAG07934.1|AE004868_5|gnl|PseudoCAP|PA4546
TIGR NTL03PA04547
UniParc UPI0000131ACF
UniProtKB Acc P33639
UniProtKB ID PILS_PSEAE
UniRef100 UniRef100_P33639
UniRef50 UniRef50_P33639
UniRef90 UniRef90_P33639

Product

Feature Type CDS
Coding Frame 1
Product Name
two-component sensor PilS
Product Name Confidence: Class 1
Synonyms protein kinase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -2.04
Kyte-Doolittle Hydrophobicity Value 0.014
Molecular Weight (kDa) 59.0
Isoelectric Point (pI) 6.71

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Cytoplasmic Membrane Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database VFDB : VF0082
Category Type IV pili biosynthesis
Evidence
Host Organism
Notes
Reference

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 620 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004208 (433 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4546
Search term: pilS

Human Homologs

References

Dual function of PilS during transcriptional activation of the Pseudomonas aeruginosa pilin subunit gene.
Boyd JM, Lory S
J Bacteriol 1996 Feb;178(3):831-9
PubMed ID: 8550520
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295