Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA4827
|
Name |
Synonym: nat |
Replicon | chromosome |
Genomic location | 5418899 - 5419738 (- strand) |
Transposon Mutants | 2 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 1 transposon mutants in orthologs |
Comment | PA4827 has been cloned, expressed, characterised and crystallised. The crystal structure (PDB 1w4t) shows very similar fold to the nat protein from M. smegmatis, with rmsd of 1.3 Angstroms over 240 equivalent atoms. |
RefSeq | NP_253514.1 |
GI | 15600020 |
Affymetrix | PA4827_at |
Entrez | 882169 |
GenBank | AAG08212.1 |
INSDC | AAG08212.1 |
NCBI Locus Tag | PA4827 |
protein_id(GenBank) | gb|AAG08212.1|AE004896_2|gnl|PseudoCAP|PA4827 |
TIGR | NTL03PA04828 |
UniParc | UPI00000C5E28 |
UniProtKB Acc | Q9HUY3 |
UniProtKB ID | Q9HUY3_PSEAE |
UniRef100 | UniRef100_Q9HUY3 |
UniRef50 | UniRef50_Q9HUY3 |
UniRef90 | UniRef90_Q9HUY3 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
arylamine N-acetyltransferase
|
Synonyms |
NAT |
Evidence for Translation | |
Charge (pH 7) | -5.89 |
Kyte-Doolittle Hydrophobicity Value | -0.121 |
Molecular Weight (kDa) | 31.2 |
Isoelectric Point (pI) | 5.82 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
1W4T | TRANSFERASE | 07/29/04 | X-ray crystallographic structure of Pseudomonas aeruginosa arylamine N-acetyltransferase | Pseudomonas aeruginosa | 1.95 | X-RAY DIFFRACTION | 100.0 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 117 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG004459 (336 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA4827
Search term: arylamine N-acetyltransferase
|
Expression, purification, characterization and structure of Pseudomonas aeruginosa arylamine N-acetyltransferase.
Westwood IM, Holton SJ, Rodrigues-Lima F, Dupret JM, Bhakta S, Noble ME, Sim E
Biochem J 2005 Jan 15;385(Pt 2):605-12
PubMed ID: 15447630
|
Involvement of Cys69 residue in the catalytic mechanism of N-hydroxyarylamine O-acetyltransferase of Salmonella typhimurium. Sequence similarity at the amino acid level suggests a common catalytic mechanism of acetyltransferase for S. typhimurium and higher organisms.
Watanabe M, Sofuni T, Nohmi T
J Biol Chem 1992 Apr 25;267(12):8429-36
PubMed ID: 1569093
|
Cloning and characterization of arylamine N-acetyltransferase genes from Mycobacterium smegmatis and Mycobacterium tuberculosis: increased expression results in isoniazid resistance.
Payton M, Auty R, Delgoda R, Everett M, Sim E
J Bacteriol 1999 Feb;181(4):1343-7
PubMed ID: 9973365
|