Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA4922
|
Name |
azu
|
Replicon | chromosome |
Genomic location | 5521664 - 5522110 (- strand) |
Transposon Mutants | 2 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 2 transposon mutants in orthologs |
RefSeq | NP_253609.1 |
GI | 15600115 |
Affymetrix | PA4922_azu_at |
DNASU | PaCD00008130 |
Entrez | 878046 |
GenBank | AAG08307.1 |
INSDC | AAG08307.1 |
NCBI Locus Tag | PA4922 |
protein_id(GenBank) | gb|AAG08307.1|AE004905_5|gnl|PseudoCAP|PA4922 |
TIGR | NTL03PA04923 |
UniParc | UPI00001266B8 |
UniProtKB Acc | P00282 |
UniProtKB ID | AZUR_PSEAE |
UniRef100 | UniRef100_P00282 |
UniRef50 | UniRef50_P00282 |
UniRef90 | UniRef90_P00282 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
azurin precursor
|
Synonyms | |
Evidence for Translation |
Detected by two-dimensional gel electrophoresis and mass spectral analysis(PMID:21863142).
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
Charge (pH 7) | -0.14 |
Kyte-Doolittle Hydrophobicity Value | -0.067 |
Molecular Weight (kDa) | 16.0 |
Isoelectric Point (pI) | 6.93 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
1E65 | ELECTRON TRANSPORT | 08/08/00 | Azurin from Pseudomonas aeruginosa, apo form | PSEUDOMONAS AERUGINOSA | 1.85 | X-RAY DIFFRACTION | 100.0 |
8F5L | ELECTRON TRANSPORT | 11/14/22 | Azurin from Pseudomonas aeruginosa, Y72F/Y108F/F110L mutant | Pseudomonas aeruginosa | 1.15 | X-RAY DIFFRACTION | 97.3 |
1E67 | ELECTRON TRANSPORT | 08/09/00 | Zn-Azurin from Pseudomonas aeruginosa | PSEUDOMONAS AERUGINOSA | 2.14 | X-RAY DIFFRACTION | 100.0 |
4HZ1 | ELECTRON TRANSPORT | 11/14/12 | Crystal Structure of Pseudomonas aeruginosa azurin with iron(II) at the copper-binding site. | Pseudomonas aeruginosa | 2.2 | X-RAY DIFFRACTION | 100.0 |
5AZU | ELECTRON TRANSPORT(COPPER BINDING) | 06/23/93 | CRYSTAL STRUCTURE ANALYSIS OF OXIDIZED PSEUDOMONAS AERUGINOSA AZURIN AT PH 5.5 AND PH 9.0. A PH-INDUCED CONFORMATIONAL TRANSITION INVOLVES A PEPTIDE BOND FLIP | Pseudomonas aeruginosa | 1.9 | X-RAY DIFFRACTION | 100.0 |
4AZU | ELECTRON TRANSPORT(COPPER BINDING) | 06/23/93 | CRYSTAL STRUCTURE ANALYSIS OF OXIDIZED PSEUDOMONAS AERUGINOSA AZURIN AT PH 5.5 AND PH 9.0. A PH-INDUCED CONFORMATIONAL TRANSITION INVOLVES A PEPTIDE BOND FLIP | Pseudomonas aeruginosa | 1.9 | X-RAY DIFFRACTION | 100.0 |
1VLX | ELECTRON TRANSPORT | 10/08/96 | STRUCTURE OF ELECTRON TRANSFER (COBALT-PROTEIN) | Pseudomonas aeruginosa | 1.9 | X-RAY DIFFRACTION | 100.0 |
1JZH | ELECTRON TRANSPORT | 09/16/01 | Pseudomonas aeruginosa Azurin Ru(tpy)(bpy)(His83) | Pseudomonas aeruginosa | 1.7 | X-RAY DIFFRACTION | 100.0 |
4JKN | ELECTRON TRANSPORT | 03/10/13 | Mercury Metallated Pseudomonas aeruginosa Azurin at 1.54 A | Pseudomonas aeruginosa | 1.536 | X-RAY DIFFRACTION | 100.0 |
8F5K | ELECTRON TRANSPORT | 11/14/22 | Azurin from Pseudomonas aeruginosa, Y72F/Y108F/F110A mutant | Pseudomonas aeruginosa | 1.25 | X-RAY DIFFRACTION | 97.3 |
Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.
Drug Name | Source Accession | Source DB | Version | Target Accession | Target Description | Percent Identity | Alignment Length | E-Value |
Tris-Hydroxymethyl-Methyl-Ammonium | DB03570 | DrugBank | 5.1.4 | P00282 | Azurin | 100.0 | 148 | 1.2e-78 |
1-[Pyrrol-1-Yl-2,5-Dione-Methoxymethyl]-Pyrrole-2,5-Dione | DB04085 | DrugBank | 5.1.4 | P00282 | Azurin | 100.0 | 148 | 1.2e-78 |
lambda-bis(2,2'-bipyridine)imidazole ruthenium (II) | DB03871 | DrugBank | 5.1.4 | P00282 | Azurin | 100.0 | 148 | 1.2e-78 |
Tetra(Imidazole)Diaquacopper (I) | DB04231 | DrugBank | 5.1.4 | P00282 | Azurin | 100.0 | 148 | 1.2e-78 |
1,1'-HEXANE-1,6-DIYLBIS(1H-IMIDAZOLE) | DB06968 | DrugBank | 5.1.4 | P00282 | Azurin | 100.0 | 148 | 1.2e-78 |
(1,10 Phenanthroline)-(Tri-Carbon Monoxide) Rhenium (I) | DB04100 | DrugBank | 5.1.4 | P00282 | Azurin | 100.0 | 148 | 1.2e-78 |
lambda-bis(2,2'-bipyridine)imidazole osmium (II) | DB03492 | DrugBank | 5.1.4 | P00282 | Azurin | 100.0 | 148 | 1.2e-78 |
S-Hydroxycysteine | DB01915 | DrugBank | 5.1.4 | P00282 | Azurin | 100.0 | 148 | 1.2e-78 |
Tetra(Imidazole)Diaquacopper (Ii) | DB03840 | DrugBank | 5.1.4 | P00282 | Azurin | 100.0 | 148 | 1.2e-78 |
4,7-Dimethyl-[1,10]Phenanthroline | DB02586 | DrugBank | 5.1.4 | P00282 | Azurin | 100.0 | 148 | 1.2e-78 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 57 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG004539 (509 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA4922
Search term: azu
Search term: azurin precursor
|
Proteome analysis reveals adaptation of Pseudomonas aeruginosa to the cystic fibrosis lung environment.
Sriramulu DD, Nimtz M, Romling U
Proteomics 2005 Sep;5(14):3712-21
PubMed ID: 16097035
|
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
|
Type 1, blue copper proteins constitute a respiratory nitrite-reducing system in Pseudomonas aureofaciens.
Zumft WG, Gotzmann DJ, Kroneck PM
Eur J Biochem 1987 Oct 15;168(2):301-7
PubMed ID: 3665926
|
The azurin gene from Pseudomonas aeruginosa. Cloning and characterization.
Arvidsson RH, Nordling M, Lundberg LG
Eur J Biochem 1989 Jan 15;179(1):195-200
PubMed ID: 2537198
|
Complex formation between the copper protein, azurin and the cytochrome c peroxidase of Pseudomonas aeruginosa.
Brittain T, Greenwood C
J Inorg Biochem 1992 Oct 1;48(1):71-7
PubMed ID: 1326600
|
Crystal structures of modified apo-His117Gly and apo-His46Gly mutants of Pseudomonas aeruginosa azurin.
Hammann C, van Pouderoyen G, Nar H, Gomis Rüth FX, Messerschmidt A, Huber R, den Blaauwen T, Canters GW
J Mol Biol 1997 Feb 21;266(2):357-66
PubMed ID: 9047369
|
Electron transfer from quinohemoprotein alcohol dehydrogenase to blue copper protein azurin in the alcohol oxidase respiratory chain of Pseudomonas putida HK5.
Matsushita K, Yamashita T, Aoki N, Toyama H, Adachi O
Biochemistry 1999 May 11;38(19):6111-8
PubMed ID: 10320337
|
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
|
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142
|