Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA4999
|
Name |
waaL
|
Replicon | chromosome |
Genomic location | 5616310 - 5617515 (+ strand) |
Transposon Mutants in orthologs | 2 transposon mutants in orthologs |
Comment | WaaL is important for cell wall integrity and motility of the bacteria. |
RefSeq | NP_253686.1 |
GI | 15600192 |
Affymetrix | PA4999_at |
Entrez | 881661 |
GenBank | AAG08384.1 |
INSDC | AAG08384.1 |
NCBI Locus Tag | PA4999 |
protein_id(GenBank) | gb|AAG08384.1|AE004912_10|gnl|PseudoCAP|PA4999 |
TIGR | NTL03PA05000 |
UniParc | UPI00000C5EBC |
UniProtKB Acc | Q9HUG6 |
UniProtKB ID | PA99_PSEAE |
UniRef100 | UniRef100_Q9HUG6 |
UniRef50 | UniRef50_Q9HUG6 |
UniRef90 | UniRef90_Q9HUG6 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
O-antigen ligase, WaaL
|
Synonyms | |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
Charge (pH 7) | 15.11 |
Kyte-Doolittle Hydrophobicity Value | 0.628 |
Molecular Weight (kDa) | 44.4 |
Isoelectric Point (pI) | 10.25 |
Individual Mappings | |
Additional evidence for subcellular localization |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 15 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG004608 (840 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA4999
Search term: waaL
Search term: O-antigen ligase, WaaL
|
Functional characterization of WaaL, a ligase associated with linking O-antigen polysaccharide to the core of Pseudomonas aeruginosa lipopolysaccharide.
Abeyrathne PD, Daniels C, Poon KK, Matewish MJ, Lam JS
J Bacteriol 2005 May;187(9):3002-12
PubMed ID: 15838026
|
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
|
WaaL of Pseudomonas aeruginosa utilizes ATP in in vitro ligation of O antigen onto lipid A-core.
Abeyrathne PD, Lam JS
Mol Microbiol 2007 Sep;65(5):1345-59
PubMed ID: 17697256
|