Pseudomonas aeruginosa PAO1, PA5043 (pilN)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5043
Name
pilN
Replicon chromosome
Genomic location 5679053 - 5679649 (- strand)
Transposon Mutants 2 transposon mutants in PAO1

Cross-References

RefSeq NP_253730.1
GI 15600236
Affymetrix PA5043_pilN_at
Entrez 881025
GenBank AAG08428.1
INSDC AAG08428.1
NCBI Locus Tag PA5043
protein_id(GenBank) gb|AAG08428.1|AE004917_7|gnl|PseudoCAP|PA5043
TIGR NTL03PA05044
UniParc UPI00000D424B
UniProtKB Acc G3XD30
UniProtKB ID G3XD30_PSEAE
UniRef100 UniRef100_G3XD30
UniRef50 UniRef50_G3XD30
UniRef90 UniRef90_G3XD30

Product

Feature Type CDS
Coding Frame 1
Product Name
type 4 fimbrial biogenesis protein PilN
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 4.24
Kyte-Doolittle Hydrophobicity Value -0.303
Molecular Weight (kDa) 22.2
Isoelectric Point (pI) 10.10

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Cytoplasmic Membrane Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database VFDB : VF0082
Category Type IV pili biosynthesis
Evidence
Host Organism
Notes
Reference

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 97 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004646 (485 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5043
Search term: pilN

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Characterization of a five-gene cluster required for the biogenesis of type 4 fimbriae in Pseudomonas aeruginosa.
Martin PR, Watson AA, McCaul TF, Mattick JS
Mol Microbiol 1995 May;16(3):497-508
PubMed ID: 7565110
Characterization of genes required for pilus expression in Pseudomonas syringae pathovar phaseolicola.
Roine E, Nunn DN, Paulin L, Romantschuk M
J Bacteriol 1996 Jan;178(2):410-7
PubMed ID: 8550460
PilM/N/O/P proteins form an inner membrane complex that affects the stability of the Pseudomonas aeruginosa type IV pilus secretin.
Ayers M, Sampaleanu LM, Tammam S, Koo J, Harvey H, Howell PL, Burrows LL
J Mol Biol 2009 Nov 20;394(1):128-42
PubMed ID: 19857645